烟草核心种质库构建及遗传多样性研究(英文)  

Genetic diversity and development of core collection in tobacco(Nicotiana tabacum L.)resources

作　　者：周佳萍[1] 杨春元 吴春 王仁刚 史跃伟 谢升东 王志红 徐海明[1,3] 任学良 

机构地区：[1]浙江大学农业与生物技术学院生物信息学研究所,杭州310058 [2]贵州烟草科学研究院,贵阳550081 [3]浙江大学空气污染与健康研究中心,杭州310058

出　　处：《浙江大学学报（农业与生命科学版）》2014年第4期440-450,共11页Journal of Zhejiang University:Agriculture and Life Sciences

基　　金：Supported by the National Natural Science Foundation of China(No.31271608)

摘　　要：为有效地研究烟草遗传多样性和筛选潜在的繁殖材料,以贵州省烟草科学研究院805份烟草种质为实验材料,分别在贵州省金沙和福泉2个试验点进行多点田间试验,并采用混合线性模型预测12个农艺性状的基因型效应值,以构建烟草的核心种质库并研究其遗传多样性.结果表明,待预测的12个农艺性状中,11个性状有显著的基因型效应,10个性状有显著的基因型与环境互作效应.利用预测的基因型值,比较不同抽样方法、抽样比率和系统聚类方法下核心子集多样性的结果表明,在10%抽样比率下,重心法聚类结合优先取样获得的S1C3_10子集成为烟草种质库的核心子集.通过主成分分析法比较核心种质与原始群体样本分布的空间结构和特征,运用相关系数检验原始群体性状间的遗传关系是否被核心种质很好地保留.结果表明,核心种质库能很好地代表烟草农艺性状的遗传多样性.一些潜在的高产育种材料如Y177和Y178也被包含在内.综上表明,作为种质资源的一个核心子集,烟草核心种质库的构建将有利于烟草育种者对整个资源的了解,促进遗传多样性在今后烟草育种计划中的利用.Plant germplasm is an important gene resource for plant breeding and genetic improvement of traits . Core collection , as a representative subset of germplasm , provides an effective entry to investigate genetic diversity and screen potential breeding material . Most agronomic traits of tobacco often exhibit strong genetic variation which can be decomposed into genotypic and genotype × environment interaction variations . A tobacco collection of 805 accessions were evaluated in two locations , Fuquan and Jinsha City of Guizhou Province , China . The genetic variations of 12 agronomic traits were analyzed and the genotypic values of each accession were predicted by a mixed linear model approach . Significant genotypic variations were detected for 11 traits , as well as genotype × environment interaction for 10 traits . Based on the predicted genotypic values , the combination strategies of sampling and clustering methods in the procedure of stepwise clustering for constructing core collection were screened in terms of magnitude of genetic variation captured by sampled subsets . Finally , the subset （ S1C3_10） , sampled by a centroid method combined with preferred sampling at 10% proportion , was determined to be a core collection of the tobacco germplasm . The representative and validation of the core collection was examined visually by the accession distribution pattern in the plot established by first two principal components , as well as by the correlation coefficients in terms of magnitude and significance between the core and the initial collections . The results showed that the initial collection was well represented by the core collection , and some potential breeding materials for high yield breeding program of tobacco , such as Y 177 and Y178 , were included in the core collection . In sum , the constructed core collection will facilitate tobacco breeder to access to whole resources and promote the utilization of genetic diversity in future breeding program of tobacco in China .

关 键 词：烟草 遗传多样性 核心种质 基因与环境互作 

分 类 号：S572[农业科学—烟草工业] S32[农业科学—作物学]