虫瘟霉属真菌中国一新纪录种——西虫瘟霉的分离和鉴定  

A New Zoophthora Occidentalis Pathogen of Myzus Persicae in China

作　　者：伍开亮[1] 黄志宏[1] 陈思涵[1] 

机构地区：[1]华侨大学生物工程与技术系,福建厦门361021

出　　处：《福建农业学报》2014年第7期673-677,共5页Fujian Journal of Agricultural Sciences

基　　金：福建省自然科学基金项目(2013J01133);中央高校基本科研专项(JB-ZR1116);华侨大学科研基金项目(09BS402)

摘　　要：从福建省邵武市的桃蚜上分离出1株病原真菌，通过形态学鉴定为西虫瘟霉Zoophthora occidentalis。其初生分生孢子长椭圆形，有明显的孢领，大小为（23.4～31.2）μm×（7.8～10.9）μm，L/D＝2.4～3.8。次生分生孢子与初生分生孢子同形，大小为（15.6～25.4）μm×（7.8～10.4）μm，L/D＝2.46～4.08。毛管孢子大小为（20.8～29.9）μm×（6.0～10.4）μm，L/D＝2.4～3.6，柄长36.4～104.0μm。菌丝直径6.5～8.8μm。休眠孢子表面光滑，无色透明，直径23.9～29.9μm。用“孢子浴”方法将该菌对桃蚜接种，结果显示接种后第4d为死亡高峰期，最终累计死亡率5.4％～95.7％，因剂量而异。经时间-剂量-死亡率模型分析，测得接种4d后LD50低于10个孢子·mm-2，表明该菌株对桃蚜具有较强的毒力。A newly discovered fungal pathogen isolated from the green peach aphids ,Myzus persicae ,in Fujian was identified as Zoophthora occidentalis . Its elongate ovoid primary conidia sized （23.4 -31.2） μm × （7.8 -10.9）μm （L/D = 2.4-3.8） and had a clear spore collar .Secondary conidia liked primary （15.6-25.4） μm × （7.8-10.4） μm （L/D = 2.46 -4.08） was also oblong in shape （15.6 -25.4） μm × （7.8 -10.4） μm with an L/D ratio of 2.46-4.08 . The size of its capilliconidium was （20.8 -29.9） μm × （6.0 -10.4） μm （L/D = 2.4 -3.6） ,and the stipe was 36.4-104.0 μm long . The diameter of its mycelium was 6.5-8.8 μm . Its resting spores were smooth ,colorless and transparent with a diameter ranging from 23.9 μm to 29.9 μm . Most of the infected aphids were killed in the 4th day , and green peach aphids to induce a peaked death rate in 4 days and cumulative mortality ranging from 5.4% to 95.7% ,which increased with the spore concentration . The resulting data fitted well on a time-dose-mortality model . The LD50 after 4 days estimated by the model was less than 10 conidias · mm -2 ,indicating the entomophthoralean fungus to be a potent pathogen for M . persicae .

关 键 词：西虫瘟霉 桃蚜 生物测定 虫霉目 

分 类 号：Q949[生物学—植物学]