HPLC同时测定白花丹中白花丹醌及香草酸的含量  

Determination on the Content of Plumbagin and Vanillic Acid in Plumbago by HPLC Concurrently

作　　者：刘鼎[1] 钟鸣 余胜民 陈勇[1] 

机构地区：[1]广西中医药大学 [2]广西民族医药研究院,530023

出　　处：《广西中医药》2014年第4期70-73,共4页Guangxi Journal of Traditional Chinese Medicine

基　　金：国家科技支撑计划(项目编号:2012BAI27B06-4);广西中药药效研究重点实验室2010年度自主研究课题(合同编号:10-046-04-Z8);广西自然科学基金创新研究团队项目"中药新药基础研究"(合同编号:2011GXNSFF018006)

摘　　要：目的：建立同时测定白花丹中白花丹醌和香草酸含量的方法,为药材的质量评价提供科学依据.方法：采用高效液相色谱法,以甲醇-0.15％磷酸为流动相进行梯度洗脱,检测波长为260 nm,流速为1.0 ml/min.结果：白花丹醌在9.95～199μg范围内与峰面积值呈现良好的线性关系,r=0.9998,平均回收率为99.52％;香草酸在10.1～202 μg范围内与峰面积值呈现良好的线性关系,r=0.9997,平均回收率为99.58％;白花丹中白花丹醌平均含量为0.0310％,香草酸平均含量为0.0124％.结论：本法简便、准确,灵敏度高,重复性好,可用于控制白花丹药材的质量.Objective： To establish the approach for determining content of plumbagin and vanillic acid in plumbago, so as to provide scientific basis for the quality assessment of medicinal material. Method： HPLC with methanol-0.15% phosphoric acid as the mobile phase gradient elution was applied. Detection wavelength was of 260 nm, the flow rate of 1.0ml/min. Result： Plumbagin showed a good linear relationship with peak area values in the range of 9.95-199 ug, r=0.9998. The average recovery was 99.52%. Vanilla acid presented a good linear relationship with peak area values in the range of 10.1-202 ug, r=0.9997. The average recovery was 99.58%. The content of plumbagin and vanillic acid in plumbago was 0.0310% and 0.0124% respectively. Conclusion： The approach was simple and accurate with high sensitivity and good reproducibility. It can be use to control the quality of plumbago.

关 键 词：白花丹 白花丹醌 香草酸 高效液相色谱法 

分 类 号：R284.1[医药卫生—中药学]