维C和左氧氟沙星对白蛋白的荧光淬灭研究  

The Interaction between Levofloxacin and Bovine Serum Albumin in Presence of Vitamin C

作　　者：胡威[1] 高宗华[1] 黄玉玲[1] 

机构地区：[1]滨州医学院化学教研室,山东烟台264003

出　　处：《广州化工》2014年第17期54-55,138,共3页GuangZhou Chemical Industry

基　　金：山东省高校科技计划(J13LD10)

摘　　要：通过荧光光谱法研究了在维生素C存在下左氧氟沙星对牛血清白蛋白的荧光淬灭作用。在模拟生理条件(pH=7.4,37℃)下,根据Stem-Volmer方程,确定了在维生素C存在条件下,左氧氟沙星与牛血清白蛋白的淬灭类型仍为静态淬灭,左氧氟沙星对牛血清白蛋白的荧光淬灭减弱,结合常数和结合位点均变小。为研究左氧氟沙星和维生素对蛋白质构象的影响等提供了重要信息。To study the binding interaction of levofloxacin with bovine serum albumin in the presence of Vc by fluorescence spectrophotometry was studied. After analyzing the fluorescence data according to Stem- Volmer equation in physiological condition（ pH = 7. 4,37 ℃）,the type of fluorescence quenching of levofloxacin was static quenching. Vc can decrease the combining constant and binding site of levofloxacin with Bovine Serum Albumin（ BSA）,and the fluorescence quenching of levofloxacin was reduced with BSA. The results provided important information for the research of levofloxacin and configuration modification of BSA by levofloxacin and vitamin.

关 键 词：维生素C 左氧氟沙星 荧光光谱法 牛血清白蛋白 

分 类 号：O657.34[理学—分析化学]