HPLC法测定宫炎康颗粒中芍药苷的含量  

Determination of Paeoniflorin in Gongyankang Granule with HPLC

作　　者：廖九明 廖厚知 陈晓军 莫少红 阮碧芳 黄园 陆红萍 

机构地区：[1]广西博科药业有限公司,广西南宁530007

出　　处：《广州化工》2014年第17期124-126,共3页GuangZhou Chemical Industry

摘　　要：建立了宫炎康颗粒中芍药苷的含量测定方法。采用反相高效液相色谱法,YMC pack ODS-A C18色谱柱（150 mm×4.6 mm,5μm）;乙腈-0.1%磷酸溶液（12.5∶87.5）为流动相;检测波长为230 nm,流速1.0 mL·min-1。结果表明：芍药苷在0.222－2.220μg范围内与峰面积呈良好线性关系,r=0.9999,芍药苷平均加样回收率为99.19%,RSD为0.56%,精密度、稳定性良好。本方法可靠性高,分离度、准确性、重复性好,适用于宫炎康颗粒中芍药苷的含量测定。The HPLC method for determination of paeoniflorin in gongyankang granule was developed. The analytical column was YMC pack ODS- A C18（ 4. 6 mm ×150 mm,5 μm） filled with a 5 μm stationary phase. The mobile phase was a mixture of acetonitrile- 0. 1% phodphric acid（ 12. 5∶87. 5）,and the flow- rate was 1 mL·min- 1. The detector wavelength was set at 230 nm. The calibration curve was linear over the range of 0. 222 －2. 220 μg for andrographolide（ r =0. 9999）. The average recovery of paeoniflorin was 99.19%. The precision and stability were fine. The proposed method with high reliability,separation,accuracy,and repeatability may be used in the quantitative analysis of paeoniflorin in Gongyankang Granule.

关 键 词：高效液相色谱法 宫炎康颗粒 芍药苷 

分 类 号：R917[医药卫生—药物分析学]