川楝子毒性的血清代谢组学研究  

Serum metabonomics study on toxicity of Fructus Toosendan

作　　者：李晶晶[1,2] 王晓娟[1] 刘妍如[1] 杨建云[1] 肖炳坤[1] 黄荣清[1] 

机构地区：[1]军事医学科学院放射与辐射医学研究所,北京100850 [2]南昌大学药学院,南昌330006

出　　处：《国际药学研究杂志》2014年第4期468-472,共5页Journal of International Pharmaceutical Research

基　　金：国家自然科学基金资助项目(21375147,81072613);北京市自然科学基金资助项目(7142125);“十二五”国家“重大新药创制”科技重大专项综合性新药研究开发技术大平台资助项目(2012ZX09301003-001-010)

摘　　要：目的基于1HNMR技术和主成分分析(principalcomponentanalysis,PCA)方法,观察给予川楝子乙酸乙酯提取物后小鼠血清代谢物组的变化,并对其毒性机制进行解释。方法给药组灌服川楝子乙酸乙酯提取物,采集血清进行1H NMR检测,利用主成分分析法对小鼠血清内源性小分子代谢产物进行分析处理。建立川楝子毒性模型并收集数据后,运用PCA载荷图对磁共振氢谱和相关代谢产物进行鉴定和分类。结果 PCA三维得分图中给药组与对照组无明显重叠部分,载荷图中显示最大变异代谢物,生物标志物变化明显,发现小鼠血清中参与能量代谢的葡萄糖和乳酸含量增加,甘氨酸等氨基酸水平明显上升,胆碱水平下降。结论结合小鼠体质量和血清生化指标,显示川楝子乙酸乙酯提取物具有肝肾毒性。Objective 1H NMR spectroscopy and principal component analysis（PCA） were used to investigate the mouse serum metabonomics and toxic mechanism after oral administration of Fructus Toosendan. Methods In this study, mice were orally administered with the ethyl acetate extract of Fructus Toosendan before collecting serum samples. Then 1H NMR was utilized to identify the metabolites with the PCA. After the experimental model of toxicity was established and the data were collected, NMR spectral regions and the metabolites were identified and ranked according to their weight aline with the loading plot. Results The scores plot for classification within groups was clear and showed a great difference from the control group. Combining the body weight and biochemical results, the elevated levels of glucose, lactate, taurine, arginine, glycine and decreased levels of betaine, creatine, amino and neurotransmitters were observed. Conclusion Our current study indicated that the ethyl acetate extract of Fructus Toosendan has liver and kidney toxicity.

关 键 词：代谢组学 ^1H NMR 川楝子 血清 

分 类 号：R285.5[医药卫生—中药学]