核桃楸皮抗衰老的自由基机制研究  

Study on Anti-aging Free Radical Mechanism of Walnut Skin

作　　者：崔红[1] 范景辉[1] 金春花[1] 

机构地区：[1]牡丹江医学院红旗医院,黑龙江牡丹江157011

出　　处：《中国食物与营养》2014年第8期71-73,共3页Food and Nutrition in China

基　　金：黑龙江省卫生厅科研课题"核桃楸皮抗衰老的自由基机制研究"(项目编号:2011-300)

摘　　要：目的:对核桃楸皮抗衰老的自由基机制进行研究。方法:选择ICR小鼠共60只,雌雄各半,使用四唑盐微量自动比色法测定脾淋巴细胞转化指数;免疫荧光法测定脾T淋巴细胞亚群;用PI染色法测定脾淋巴细胞凋亡率,观察小鼠各项免疫功能的影响。结果:实验组小鼠大脑组织匀浆液全部>15,只有正常组大鼠正常(P<0.05)。结论:通过对核桃楸皮抗衰老的自由基机制进行研究发现,核桃楸皮具备抗衰老作用,为临床研究抗衰老新药提供了科学依据。[Objective] To study the free radical mechanism of walnut skin anti-aging. [Methods] Totally 60 ICR mice were chosen, in which male and female were both 30. Spleen lymphocyte transformation index was determinated using the four salts automated colorimetric microassay, spleen T lymphocyte subsets was determined using immunofluorescence, spleen lymphocyte apoptosis rate was determined by PI staining and then we observed the effect on the immune function of mice. [Result] The experimental group mice brain tissue homogenate were all above 15, only the normal group rats were normal （P 〈 0. 05） . [Conclusion] Juglans mandshurica have anti-aging effect, for the clinical study of anti aging drugs and natural non-toxic provides effective basis.

关 键 词：核桃楸皮 抗衰老 自由基机制 

分 类 号：R285.5[医药卫生—中药学] TS255.6[医药卫生—中医学]