黄喉拟水龟腐皮病病原菌的分离鉴定及药敏试验  

Isolation,Identification and Antimicrobial Sensitivity Test of Bacterial Pathogens in Turtle Mauremys mutica with Skin-rotting Disease

作　　者：兰云[1] 胡秀彩[1] 沈晓静[1] 李雪[1] 吕爱军[1] 朱爱华[1] 冯照军[1] 

机构地区：[1]江苏师范大学生命科学学院,江苏徐州221116

出　　处：《动物医学进展》2014年第9期127-131,共5页Progress In Veterinary Medicine

摘　　要：分离鉴定黄喉拟水龟腐皮病致病菌,以了解其致病性和药敏特性。通过形态学观察、生理生化特性、16SrDNA序列扩增及系统发育树分析等方法系统鉴定,并进行动物感染试验、药敏试验。结果从发病黄喉拟水龟体表病灶分离到一株革兰阴性短杆菌,编号为TSM13032菌株,PCR扩增获得16SrDNA序列片段大小为1 482bp(登录号:KF611897),该分离菌与人源类香味菌属(Myroides sp.)参考菌株基因序列同源性达99.92%;系统发育树分析表明,TSM13032菌株与Myroides sp.(登录号:JX966100)亲缘关系最近,自然聚类为一支;动物感染试验表明对稚龟有致病性;对氨苄西林、哌拉西林、头孢哌酮、头孢噻吩、头孢孟多、阿奇霉素、萘啶酸、氯霉素、磺胺异恶唑等9种抗生素高度敏感。初步鉴定该菌为类香味菌属新种(Myroides sp.),首次报道黄喉拟水龟腐皮病病原与类香味菌有关。To isolate and identify the pathogenic bacteria in turtle Mauremys mutica with skin-rotting dis-ease,and analyse the pathogenicity and drug sensitivity of the bacteria,the bacterial pathogens were isola-ted from the skin lesions of diseased Mauremys mutica ,and then identified by physiological and biochemi-cal characteristics,16 S rDNA sequencing,construction and analysis of phylogenetic tree;Furthermore, the drug sensitivity test and animal infection test were carried out by using standard methods.A novel Gram-negative,short rod-shaped bacterium,strain TSM13032,was isolated from the skin lesions of dis-eased turtle,and a 1 482 bp 16 S rDNA fragment of the isolate was obtained （GenBank accession number KF611897）,the sequence homology of 16 S rDNA was 99.92% with Myroides sp.（GenBank accession number JX966100）from humans,the phylogenetic tree analysis showed that TSM13032 strain was clus-tered closely with newly reported bacterial isolate Myroides sp.;The pathogenicity to baby turtles was as-sessed by animal infection test.The antimicrobial sensitivity analysis showed that the isolate was highly sensitive to ampicillin,piperacillin,cefoperazone,cephalothin,cefamandole,azithromycin,nalidixic acid, chloramphenicol and sulfisoxazole.A newly isolated strain was identified as Myroides sp.,this is the first report isolated Myroides sp.from diseased Mauremys mutica .

关 键 词：黄喉拟水龟 类香味菌 分离鉴定 致病性 药敏试验 

分 类 号：S855.12[农业科学—临床兽医学]