匹伐他汀预处理对大鼠心肌缺血再灌注后炎性因子及氧化应激的影响  

Effects of pitavastatin on inflammatory factors and the role of oxidative stress in a rat model of myocardial ischemiareperfusion

作　　者：尤杨[1] 戚国庆[1] 夏岳[1] 冯倩 张庆文[1] 杨志瑜[1] 

机构地区：[1]河北医科大学第一医院心内3科,石家庄050000 [2]张家口市第一医院心内3科,075000

出　　处：《疑难病杂志》2014年第9期932-935,共4页Chinese Journal of Difficult and Complicated Cases

基　　金：河北省科学技术研究与发展计划(No.112761155)

摘　　要：目的探讨匹伐他汀预处理对大鼠急性心肌缺血再灌注后炎性因子及抗氧化物酶活性、心肌梗死面积的影响。方法成年雄性SD大鼠42只随机分为假手术组(Sham组)、对照组(I/R组)及实验组(Statin组),每组14只。Sham组与I/R组术前给予生理盐水2 ml/d灌胃4周,Statin组给予匹伐他汀0.42 mg·kg^(-1)·d^(-1),溶解成2ml灌胃4周。术后检测血清中白细胞介素-6(IL-6)、IL-26、肿瘤坏死因子(TNF)-α及磷酸肌酸激酶同工酶(CK-MB)的水平,留取结扎线下部分心肌组织用黄嘌呤氧化酶法测心肌总超氧化物岐化酶(T-SOD)活性,用氯化三苯基四氮唑(TTC)、伊文氏蓝(Evans blue)双染色法测定各组心肌梗死面积及缺血区面积。结果与I/R组比较,Statin组心肌梗死面积(AN/AAR)明显减少(P<0.01);与Sham组相比,I/R组、Statin组的血清IL-6、IL-26、TNF-α及CK-MB含量显著升高(P<0.01),T-SOD含量下降(P<0.01);与I/R组比较,Statin组IL-6、IL-26、TNF-α及CK-MB含量较低(P<0.05),T-SOD含量较高(P<0.01)。结论匹伐他汀可有效抑制炎性介质释放,提高机体抗氧化能力,减少心肌细胞坏死数量,减轻再灌注后心肌坏死程度,对预防缺血再灌注心肌损伤有积极作用。Objective To explore the effects of pitavastain on inflammatory factors （ IL-6,IL-26,TNF-α） and T-SOD and cardiac infarct area in a rat model of myocardial ischemia-reperfusion , which will provide a theoretical basis for pitavastain clinical application in myocardial ischemia-reperfusion .Methods A total of 42 male SD rats were randomly assigned to three groups：Sham group , control group （ I/R group ） and experimental group （ Statin group ） .In Sham group and control group , 2ml saline were irrigated into stomach daily for 4 weeks, 0.42mg/kg pitavastain dissolved in 2ml saline were given in experi-mental group.In surgery,we weared line under the coronary artery without ligation in Sham group , and ligated the line for 30 minutes and then release it for 2 hours in I/R group and Statin group.The levels of IL-6,IL-26,TNF-αand CK-MB were dis-cerned after operation.Results Compared with I/R group,Statin group significantly reduced infarct size （AN/AAR）（ P 〈0.01）.The levels of IL-6,IL-26,TNF-αand CK-MB in control group and experimental group elevated significantly compared with Sham group（ P 〈0.01）, but lower when comes to T-SOD content（ P 〈0.05）.The levels of IL-6,IL-26,TNF-αand CK-MB in I/R group were significantly higher than Statin group （ P 〈0.05 ） .Conclusion Pitavastatin could inhibit the re -lease of inflammatory mediators ang increase antioxidant capacity .It took a positive role in ischemia-reperfusion myocardial in-jury prevention .

关 键 词：心肌缺血再灌注损伤 匹伐他汀 炎性因子 心肌梗死面积 大鼠 

分 类 号：R965[医药卫生—药理学]