16种中药颗粒剂对5种耐药菌株的体外抑菌效果比较  

In vitro antibacterial effect of 16 kinds of Chinese medicine granules on 5 kinds of drug resistant strains

作　　者：李蔼文[1] 沈素晶[1] 周兵[1] 

机构地区：[1]广东省第二中医院检验科,广州510095

出　　处：《国际医药卫生导报》2014年第18期2867-2871,共5页International Medicine and Health Guidance News

摘　　要：目的 探讨黄芩等16种中药颗粒剂对耐甲氧西林金黄色葡萄球菌（MRSA）等5种临床常见耐药菌的体外抑菌效果.方法 通过对中药药源的深入调查,选用16种具有消炎抗菌作用的中药颗粒剂,采用K-B纸片琼脂扩散法分别测量5种临床常见耐药菌的抑菌圈大小,同时各选用一种广谱抗生素作阳性对照,采用t检验与颗粒剂进行比较.结果 黄芩抑菌效果最好,耐甲氧西林金黄色葡萄球菌对其高度敏感,其他4种菌敏感度较低,抑菌圈直径在6～ 19mm之间;连翘、薄荷抑菌效果次之;板蓝根、金银花、菊花、土茯苓、大青叶、地丁、知母7种颗粒剂对这5种耐药菌没有抑菌效果;对于耐甲氧西林金黄色葡萄球菌（MRSA）,黄芩与万古霉素比较差异无统计学意义（P＞0.05）,其余颗粒剂均差于万古霉素（P＜0.05）;对于耐药绿脓杆菌,连翘与亚胺培南比较差异无统计学意义（P＞0.05）,其余颗粒剂均差于亚胺培南（P＜0.05）;对剩下的3种耐药杆菌,颗粒剂均差于亚胺培南（P＜0.05）.结论 部分中药颗粒剂对5种临床常见耐药菌有抑菌效果.Objective To explore the in vitro antibacterial effect of scutellaria baicalensis etc. 16 kinds of Chinese herbal medicine granules on methicillin-resistant staphylococcus aureus （MRSA） etc. 5 common clinical drug resistant bacteria. Methods Basing on the research on traditional Chinese medicine source, 16 kinds of traditional Chinese herbal medicine granules with anti-inflammatory and antibacterial effects were chosen. K-B disc agar diffusion method was used to measure the size of inhibition ring of 5 kinds clinical common resistant bacteria. Meanwhile, a broad-spectrum antibiotic was selected as a positive control, t test was used to compare with the granule. Results Baicalin had best antibacterial effect; methicillin resistant Staphylococcus aureus was highly sensitive to it but lowly sensitive to the other 4 kinds; the diameter of inhibition zone was 6-19 mm. Forsythia and mint came behind it in inhibitory effect. Isatis root, honeysuckle, chrysanthemum, Tuckahoe, Folium Isatidis, Herba Violae, and Anemarrhena granules had no inhibitory effect on the five kinds of drug resistant bacteria. For methicillin-resistant Staphylococcus aureus （MRSA）, Scutellaria haicalensis didn＇ t statistically differed from vancomycin （P 〉 0.05）, while the rest of the granules were worse than vancomycin （P 〈 0.05）. For resistant Pseudomonas aeruginosa, Fructus Forsythiae didn＇ t statistically differed from imipenem （P 〉 0.05）, while the rest of the granules were worse than imipenem （P 〈 0.05）. For the remaining 3 resistant bacilli, granules were worse than imipenem （P 〈 0.05）. Conclusion Part of the traditional Chinese medicine granules have inhibitory effect on the 5 clinical common resistant bacteria.

关 键 词：颗粒剂 K-B纸片琼脂扩散法 耐药菌株 

分 类 号：R446.5[医药卫生—诊断学]