奶牛结核病两种检测方法的比较试验  

Comparison Tests of Two Kinds of Methods for Detection of Bovine Tuberculosis

作　　者：杨莉[1] 刘付强 宋大书 孙启跃[1] 田孟林 吴位珩[1] 杨茂生[1] 

机构地区：[1]贵州省畜牧兽医研究所,贵州贵阳550005 [2]贵州省清镇市畜牧局草地奶牛示范场,贵州清镇551400

出　　处：《贵州畜牧兽医》2014年第4期16-18,共3页Guizhou Journal of Animal Husbandry & Veterinary Medicine

基　　金：贵州省农业攻关项目[黔科合NY(2009)3086];贵州省畜禽健康养殖技术创新能力建设项目[黔科合院所创能(2010)4004];贵州省农委项目"动物疫病综合防治技术推广应用及研究-奶牛结核PCR快速诊断试剂盒研发与推广应用"

摘　　要：采用自制的牛结核病PCR快速诊断试剂盒,对某奶牛场100头份奶牛奶样进行检测,检出阳性牛16头;采用结核菌素皮内注射法(PPD)检出阳性牛17头,结果符合率为94.11%。用牛结核病PCR快速诊断试剂盒检测所需时间为6 h,显示其快速、特异等优点,为今后牛结核病的检疫工作提供了1个新方法。PCR was developed for detecting Mycobacterium bovis from 100 samples of mlik. The result showed 16 milk samples were infected with PCR detection, and 17 sample were positive with PPD, and the coincidence rate was 94.11%. It took 6 h with PCR checking Bovine tuberculosis and can a new method for checking Mycobacterium bovis infection.

关 键 词：奶牛 结核病 检测 

分 类 号：S858.23[农业科学—临床兽医学]