4,4-二甲氧基-2-丁酮对大鼠亚急性经口毒性研究  

Subacute oral toxicity of 4,4-dimethoxy-2-butanone in rats

作　　者：杨秀鸿 张丽娜 吴军 

机构地区：[1]湖南省职业病防治院,湖南长沙410007

出　　处：《中国职业医学》2014年第4期436-440,共5页China Occupational Medicine

摘　　要：目的探讨4,4-二甲氧基-2-丁酮的亚急性毒性作用。方法 60只无特定病原体级健康成年SD大鼠随机分为对照组、低剂量组、中剂量组、高剂量组、对照附加组和高剂量附加组共6组。3个剂量组分别以150.0、300.0和600.0 mg/（kg体质量·d）玉米油-4,4-二甲氧基-2-丁酮混悬液经口灌胃染毒28 d,对照组给予等体积的玉米油,2个附加组动物染毒28 d后停止给药,继续观察14 d后处理。观察动物的中毒表现和组织器官的病理组织学改变,分析染毒末期动物血常规和血液生化指标变化。结果染毒第8-15天后,高剂量组和高剂量附加组动物相继出现少动、精神不振和消瘦等中毒症状,持续至染毒结束。6组大鼠在各时间点体质量差异均有统计学意义（P〈0.01）;不同剂量组与时间点均有交互作用（P〈0.01）。第28天高剂量组雌性和雄性大鼠体质量低于同性别对照组（P〈0.05）,第7、21、28、35和42天,高剂量附加组雌性大鼠体质量分别低于同性别对照附加组（P〈0.05）。高剂量组雌性和雄性大鼠总食物利用率分别低于同性别对照组（P〈0.01）。高剂量附加组雌性和雄性大鼠总食物利用率分别低于同性别对照附加组（P〈0.01,P〈0.05）。中、高剂量组雌性大鼠总蛋白（TP）和白蛋白（Alb）水平高于同性别对照组（P〈0.05）,高剂量组雌性大鼠总胆固醇水平高于同性别对照组（P〈0.01）,高剂量组雌性大鼠肌酐水平低于同性别对照组（P〈0.01）;高剂量组雄性大鼠TP和Alb水平高于同性别对照组（P〈0.05,P〈0.01）。高剂量组雌性大鼠肝、肾脏器系数分别高于同性别对照组（P〈0.05,P〈0.01）;中、高剂量组雄性大鼠肝脏脏器系数高于同性别对照组（P〈0.05）。结论本实验条件下4,4-二甲氧基-2-丁酮对大鼠具有亚急性毒性作用,能抑制其生长发育,损害其肝脏,上述损害在停止染毒后具有可逆性。雌性和�Objective To explore the subacute oral toxicity of 4,4-dimethoxy-2-butanone in SD rats. Methods Sixty healthy specific pathogen free SD rats were randomly divided into 6 groups, the control group, the low-, medium-, high- dose groups, control supplementary group and high-dose supplementary group with 10 rats, male and female half and half in each group. The dose group exposed to 4,4-dimethoxy-2-butanone was at the concentrations of 150. 0,300. 0 and 600. 0 mg/（kg bw · d） by gavage for 28 days. The control group was given the same volume of corn oil. Supplementary groups continued to be observed for 14 days after the period of the 28-day dosing. The toxic symptoms and histopathology were observed. The changes of routine blood and blood biochemical indicators were detected and analyzed. Results It showed that obvious clinical symptoms such as hyperspasmia, poor spirit and leptystic firstly appeared on day 8 to day 15 in the high-dose group and high-dose supplementary group. The differences of body weights among 5 time points and 7 time points were statistically significant （P 〈0. 01 ）, with category and time interactions （P 〈0. 01 ）. On day 28, the body weight of the high-dose group were lower than that of the control group （ P 〈 0. 05）. On day 7, 21, 28 , 35 and 42, female body weights of the high-dose supplementary group were flower than those of the same gender of the control supplementary group （ P 〈 0. 05 ）. The total food utilization of the high-dose group was lower than that of the control （ P 〈 0. 01, P 〈 0. 05 ）. The total food utilization of the high-dose supplementary group was lower than that of the control supplementary group （ P 〈 0. 01, P 〈0. 05）. The female total protein （TP） and albumin （Alb） level in medium-, high-dose groups were higher than those of the same gender of the control （ P 〈 0. 05 ）. The female total cholesterol level of the high-dose group was higher than that of the same gender of the control （ P 〈 0. 01 ）. The female

关 键 词：4 4-二甲氧基-2-丁酮 亚急性 最大无作用剂量 最小有作用剂量 大鼠 

分 类 号：R135.1[医药卫生—劳动卫生] R996[医药卫生—公共卫生与预防医学]