泰国巨竹试管快繁研究  

In vitro micropropagation of Gigantochloa tekserah

作　　者：周玲[1] 王晓芹[1] 林新春[1] 

机构地区：[1]浙江农林大学亚热带森林培育国家重点实验室培育基地,浙江临安311300

出　　处：《浙江农林大学学报》2014年第5期817-820,共4页Journal of Zhejiang A&F University

基　　金：国家自然科学基金资助项目(31270677);"十二五"浙江省农业新品种选育"竹木育种协作组"课题(2012C12908-3);浙江省自然科学基金重点资助项目(Z3100366);浙江省优先主题重点农业项目(2010C12011)

摘　　要：以泰国巨竹Gigantochloa tekserah成熟种胚为材料,研究不同激素组合对泰国巨竹丛生芽增殖及生根的影响,建立其试管繁殖体系。结果表明:泰国巨竹丛生芽的最佳增殖培养基为MS(Murashige and Skoog)+3 mg·L-1BA(6-苄基腺嘌呤)+0.01 mg·L-1TDZ(噻苯隆)+0.3 mg·L-1NAA(萘乙酸),增殖系数为2.11,芽丛生长旺盛;最优生根培养基为1/2MS+3.0 mg·L-1IBA(吲哚丁酸),生根率达70%,根系发达、粗壮;试管苗移栽至等配比的泥炭、蛭石、珍珠岩的基质中,成活率达100%。To establish a tissue culture system for commercial propagation, buds induced by mature embryos were used as explants to study the effect of different hormone combinations on bud muhiplieation and root in- duction of Gigantochloa tekserah. Results showed that for bud multiplication, since the highest proliferation co- efficient was 2.11 and cluster buds grew well, Murashige and Skoog＇s （MS） medium supplemented with 3 mg.L^-1 6-benzylaminopurine （BA）, 0.01 mg.L^-1 thidiazuron （TDZ）, and 0.3 mg.L^-1 1-naphthylaeetie acid （NAA） was the optimal medium. The best rooting medium was 1/2 MS supplemented with 3 mg.L^-1 indole-3-butyrie acid （IBA） because of the rooting rate was up to 70% with long, thick roots. After transferring to an equal ratio mixture of peat, vermiculite, and perlite, the survival rate of hardening plantlets was 100%.

关 键 词：植物学 泰国巨竹 组织培养 丛生芽 植物生长调节物质 

分 类 号：S723.1[农业科学—林木遗传育种]