花生发育果实小泡运输类型与黄曲霉抗性和发育有关(英文)  

Vesicle Trafficking Patterns in Developing Peanut Fruits Related to Aspergillus flavus Resistancy and Development

作　　者：严海燕[1] 宗成志[1] 单世华[2] 

机构地区：[1]中南民族大学生命科学学院,武汉430074 [2]山东省花生研究所,青岛266100

出　　处：《中国细胞生物学学报》2014年第8期1092-1103,共12页Chinese Journal of Cell Biology

基　　金：山东花生研究所合作项目(批准号:HZY05013)资助的课题~~

摘　　要：小泡运输介导的先天免疫在植物防卫中起重要作用。采用定量PCR和生物信息学的方法,该研究揭示两种不同的小泡运输类型分别在花生黄曲霉抗性品种C20R和敏感品种TFR发育的种子中起主要作用。VAMP726和RMR是黄曲霉抗性品种C20R中主要的小泡运输组分,VSRs VTI1a,b是黄曲霉敏感品种TFR中主要的小泡运输组分。在果实发育过程中,这些小泡运输组分的转录动态在整体转录组水平分别与相应的花生黄曲霉抗性品种C20R和敏感品种TFR差异表达的一系列基因表达趋势一致。因此,我们认为两类不同组合的小泡运输分别在黄曲霉抗性品种C20R和敏感品种TFR果实发育中起着主要运输作用,与发育中转录组水平基因表达的差异一致。这种差异早在蛋白质合成结束和运输起始阶段就已经显示,导致果实代谢和发育方向的差异,造就黄曲霉抗性的不同。Innate immunity mediated by vesicle trafficking plays important roles in plant defense. In this study, using QPCR and bioinformatics methods, we demonstrated that two different distinctive vesicle trafficking patterns played major function in peanut developing seeds of A. flavus resistant strain C20R and sensitive strain TFR. We also found that VAMP726 and RMR were the preferred vesicle trafficking components in A. flavus re- sistant strain C20R, and VSRs VTIla, b in A. flavus sensitive strain TFR. During fruit developmental process, the dynamic transcription tendency of these vesicle trafficking components was consistant to that of sets of genes in the developing seeds of C20R and TFR respectively at whole genomic transcription levels. Thus, we proposed that two vesicle transport patterns existed in C20R and TFR developing seeds respectively, which was related to the expression differences at whole genomic transcription levels in the developing seeds between C20R and TFR. Such differences could begin as early as at the end of protein synthesis and at the entry of ER as the begining of transport pathway.

关 键 词：RMR VSR TIP 小泡运输 VAMP726 SNARE 花生 

分 类 号：S565.2[农业科学—作物学]