高脂饮食小鼠脂肪组织中PAI-1、FOXC2及FOXO1表达的研究  

Study of expression of PAI-1, FOXC2 and FOXO1 in adipose tissue of mice with high fat diet

作　　者：刘亚莉[1] 易佳丽 刘剑辉 牛丽凤 

机构地区：[1]辽宁医药职业学院卫生检验教研室,辽宁沈阳110101 [2]辽宁医药职业学院教务处,辽宁沈阳110101 [3]辽宁医药职业学院医学检验技术教研室,辽宁沈阳110101

出　　处：《中国医药科学》2014年第16期26-29,共4页China Medicine And Pharmacy

基　　金：辽宁省教育厅科学研究一般项目(L2011246)

摘　　要：目的研究高脂饮食喂养小鼠内脏脂肪和皮下脂肪组织中PAI-1、FOXC2及FOXO1表达水平,探讨不同类型肥胖的机制。方法 20只小鼠随机分为对照组和高脂组,分别给予正常饮食和高脂饮食喂养12周。测定血清PAI-1以及附睾周围组织及皮下脂肪中PAI-1、FOXC2及FOXO1 mRNA的表达水平。结果高脂组小鼠体质量、血清PAI-1水平均显著高于对照组。组内比较小鼠附睾周围脂肪组织PAI-1、FOXC2mRNA表达显著高于皮下脂肪组织;FOXO1mRNA表达显著低于于皮下脂肪组织,差异有统计学意义。结论正常体重小鼠和肥胖小鼠血清PAI-1水平表达不同,内脏脂肪和皮下脂肪PAI-1、FOXC2及FOXO1表达也存在差别,这种差别可能是不同类型肥胖的机制之一。Objective To study the expression level of PAI-1, FOXC2 and FOXO1 in high fat diet of visceral fat and subcutaneous fat tissue,and to investigate the mechanism of different types of obesity.Methods 20 mouse were randomLy divided into control group and high fat group, and given normal diet and high fat diet for 12 weeks. Serum PAI-1,the expression level of PAI-1, FOXC2 and FOXO1 mRNA in epididymal tissue and subcutaneous fat were determined. Results The body weight, serum PAI-1 levels of high fat group were significantly higher than those of the control group. Within the group, PAI-1,FOXC2, mRNA expression of mouse epididymal adipose tissue were significantly higher than those of the subcutaneous adipose tissue, and the expression of FOXO1mRNA of mouse epididymal adipose tissue was significantly lower than that of subcutaneous adipose tissue, with statistical significance difference. Conclusion Expression levels of serum PAI-1 are different between normal weight mice and obese mice,and expression of fat PAI-1, FOXC2and FOXO1 in visceral fat and subcutaneous are also different. This difference may be one of the mechanisms of different types of obesity.

关 键 词：血浆纤溶酶原激活物抑制物-1 FOXC2 FOXO1 

分 类 号：R589.2[医药卫生—内分泌]