水母雪莲红色细胞系类黄酮含量和相关基因表达  

Flavonoids contents and expression analysis of related genes in red cell line of Saussurea medusa

作　　者：王亚杰[1] 李厚华[1] 付婉艺[1] 高艳[1] 王冰洁[1] 李玲[1] 

机构地区：[1]西北农林科技大学林学院,陕西杨凌712100

出　　处：《生物工程学报》2014年第8期1225-1234,共10页Chinese Journal of Biotechnology

基　　金：国家林业公益性行业科研专项(No.201204308);高等学校博士学科点专项科研基金(No.20120204120006)资助~~

摘　　要：水母雪莲为中国传统名贵中药,具有散寒除湿、活血通经、抗炎、镇痛等功效,其主要药用成分为类黄酮化合物。以水母雪莲白色系愈伤组织和经低温、高糖、强光诱导得到的红色系为材料,比较不同细胞系中类黄酮活性成分、结构基因和转录因子表达的差异。结果表明,红色系中总黄酮含量为白色系的3.60倍;重要的药用成分为芦丁,其含量达到干重的0.25%,是白色系的2.40倍;红色系中花青苷含量极高,矢车菊素3-O-己糖苷和矢车菊素3-O-琥珀酰己糖苷的含量分别达到干重的0.12%和0.19%;红色系中CHS、F3'H、FNS、FLS、DFR和ANS基因的表达均明显高于白色系;红色系中转录因子MYB、bHLH和WD40的表达也均明显高于白色系,其中MYB的表达量为白色系的19.70倍,说明红色系中转录因子的高水平表达增强了结构基因的表达,进而提高了类黄酮的合成。红色系中bHLH和WD40表达水平相似,而与MYB的表达水平相差很大,推测可能在水母雪莲中bHLH和WD40两种转录因子形成二元复合体后,和MYB共同调控类黄酮合成途径中结构基因的表达。Saussurea medusa is a rare traditional Chinese medicinal herb. Besides anti-inflammatory and analgesic activities, it has effects of disinhibiting cold, dispelling dampness and promoting blood circulation. Flavonoids are the main medicinal compounds in S. medusa. Contents of flavonoids and expression of flavonoids biosynthesis related genes in white and red （induced by low temperature, high sucrose and high light） callus were analyzed. The results showed that the total flavone in red line was 3.60 times higher compared to white line. The accumulation of rutin in red line （0.25% of dry weight） was 2.40 times higher compared to white line. Anthocyanins were abundant in red line, with the contents of cyanidin 3-O-glucosidechloride and cyanidin 3-O-succinyl glycoside 0.12% and 0.19% of dry weight respectively. CHS, F3＇H, FNS, FLS, DFR and ANS genes were highly expressed in red line compared to white line. Expression of three transcription factors （MYB, bHLH and WD40） in red line was significantly higher than that in white line, especially the expression of MYB （19.70 times higher compared to white line）. These results indicated that high expression levels of transcription factors induced high expression of structural genes in red line, thereby enhancing the flavonoids biosynthesis. The expression of bHLH and WD40 was similar, whereas it was significantly different from that of MYB, indicating that bHLH and WD40 could form a binary complex to regulate expression of structural genes and flavonoids biosynthesis.

关 键 词：水母雪莲 转录因子复合体 结构基因 类黄酮生物合成 表达分析 

分 类 号：S567.239[农业科学—中草药栽培]