黄芩苷对溃疡性结肠炎患者NF-κB及磷酸化NF-κB表达的影响  

The baicalin right patients with ulcerative colitis NF-kappa B and phosphorylation of NF-kappa B expression

作　　者：于丰彦[1] 黄绍刚[2] 张海燕[2] 叶华[3] 迟宏罡[1] 邹颖[1] 吕汝西[1,4] 郑学宝[1] 

机构地区：[1]广东医学院中医研究室,广东湛江524023 [2]广东省中医院大学城分院,广东广州511495 [3]广东医学院广东天然药物研究与开发重点实验室,广东湛江524023 [4]南方医科大学中医药学院,广东广州510515

出　　处：《中医临床研究》2014年第24期14-18,共5页Clinical Journal Of Chinese Medicine

基　　金：国家自然科学基金(NO30772701);东莞市科技计划项目(NO2008108101032)

摘　　要：目的:本研究旨在探讨UC患者外周血单个核细胞NF-κB及磷酸化NF-κB及相关细胞因子在溃疡性结肠炎(UC)中的表达,并进一步探讨体外黄芩苷干预的作用。方法:应用western blotting蛋白水平检测UC患者外周血单个核细胞NF-κB p65和Phospho-NF-k B p65,用ELISA法检测血清IFN-r、IL-4、IL-6、IL-10水平及体外黄芩苷干预下的检测上述指标。结果:与健康对照组和IBS-D组相比较,p-NK-FB明显增高,NK-FB明显下降,p-NK-FB/NK-FB明显增高,差异有显著性(P<0.05);IBS-D组和健康对照组之间无显著性差异(P>0.05)。体外培养体外黄芩苷干预下,在40umol浓度干预下p-NK-FB/NK-FB较未干预前、DMSO干预、5umol干预、10umol和20umol浓度干预下下降,存在显著性差异(P<0.05)。从ELISA检测较高浓度的黄芩苷能够明显抑制IFN-r、IL-6等细胞因子,可以使IL-4、IL-10升高。结论:黄芩苷作为黄芩汤的重要组成成分之一,可能是一种潜在的免疫抑制剂,可能抑制p-NK-FB/NK-FB,调节免疫平衡而缓解溃疡性结肠炎的炎症反应,为深入研究UC的发病机制及新药开发提供理论基础。Objective： The purpose of this study is to investigate the UC patients peripheral blood mononuclear cells NF-kappaB andphosphorylation of NF-kappaB and expression of related cytokines in ulcerative colitis （UC）, and to further explore the in vitro baicalininterventioneffect. Methods： Application Western blotting protein levels in detection of patients with UC peripheral blood mononuclearcells NF-κB p65 and phospho-NF-kB p65 detected by ELISA in serum IFN-R, IL-4, IL-6, IL-10 level and in vitro baicalin interfere withthe detection of these indicators. Results： Compared with the healthy control group and the IBS-D group compared with p-NK-FBsignificantly increased the NK-FB decreased p-NK-FB/NK-FB the significantly higher, the difference was significant （P〈0.05）;nosignificant difference （P〉0.05） between the IBS-D group and the healthy control group. Cultured in vitro in vitro baicalin in theconcentration of 40umol intervention under p-NK-FB/NK-FB than non-intervention, a DMSO intervention, 5umol intervention, 10umoland 20umol concentration intervention decline, there is a significant difference （P〈0.05） . ELISA detected a higher concentration ofbaicalin notably the inhibition of IFN-r, IL-6 and other cytokines, can make IL-4, IL-10 increased. Conclusion： baicalin as one of theimportant components of HQT, may be a potential immunosuppressive agents, may inhibit p-NK-FB/NK-FB regulation of the immunebalance and relieve ulcerative colon inflammation reaction, for in-depth UC pathogenesis and development of new drugs to provide atheoretical basis.

关 键 词：溃疡性结肠炎 NF-ΚB 磷酸化NF-κB 黄芩苷 

分 类 号：R285.5[医药卫生—中药学]