甲型副伤寒沙门菌培养条件的优化  

Optimization on growth condition of Salmonella paratyphoid A

作　　者：陈国怀[1] 曹玲[1] 罗广[1] 刘晓[1] 刘大东[1] 金红燕[1] 陈刚[1] 席仲兴[1] 

机构地区：[1]兰州生物制品研究所有限责任公司甘肃省疫苗工程技术研究中心,甘肃兰州730046

出　　处：《微生物学免疫学进展》2014年第4期26-28,共3页Progress In Microbiology and Immunology

摘　　要：优化甲型副伤寒沙门菌的培养条件,提高菌体产量。方法通过单因素及正交试验,对影响甲型副伤寒沙门菌生长的培养温度、NaCl浓度和pH等条件进行优化。结果甲型副伤寒沙门菌在NaCl浓度0.75%、温度35℃、pH6.5时菌体产量最高。结论通过对甲型副伤寒沙门菌培养条件进行优化,获得较高的菌体产量,为后期诊断试剂盒的开发奠定了基础。Objective To optimize the growth condition of Salmonella paratyphoid A. Methods To study the effect for temperature ,pH and NaCl concentration on the growth of Salmonella paratyphoid A by using the method of orthogonal design. Results The optimal culture condition of Salmonella paratyphoid A is NaCl concentration 0.75% , temperature 35 ℃ and pH 6.5. Conclusion The optimal culture condition of Salmonella paratyphoid A is successfully achieved in this investigation.

关 键 词：甲型副伤寒沙门菌 优化培养 

分 类 号：Q93-33[生物学—微生物学] R378.2[医药卫生—病原生物学]