荞麦黄酮对脑缺血再灌注损伤大鼠的保护作用  

Protective effect and mechanism of buckwheat flavones on cerebral ischemia reperfusion injury in rats

作　　者：谭双[1] 李丽[1] 赵爽[1] 李永秋[1] 

机构地区：[1]唐山市工人医院,唐山063000

出　　处：《中药药理与临床》2014年第3期73-75,共3页Pharmacology and Clinics of Chinese Materia Medica

基　　金：河北省自然科学基金资助项目(C2010001795)

摘　　要：目的：观察荞麦黄酮对脑缺血再灌注大鼠损伤的影响及其可能机制。方法：SD大鼠随机分为假手术组、模型对照组、荞麦黄酮剂量组（100、200、400mg/kg）及尼莫地平组（6mg/kg）,各组灌胃给药1次/d,连续5d,末次给药2 h后,建立大鼠大脑中动脉闭塞再灌注模型,再灌注24 h后将各组大鼠进行神经行为评分,并测定大鼠脑梗死面积、脑含水量;测定血清中超氧化物歧化酶（SOD）、丙二醛（MDA）、白细胞介素-1β（IL-1β）、白细胞介素-10（IL-10）及肿瘤坏死因子-α（TNF-α）、内皮素（ET）、诱生型一氧化氮合酶（iNOS）及一氧化氮（NO）含量。结果：与假手术组比较,模型对照组大鼠神经行为评分值、脑梗死面积、脑含水量、MDA、IL-1β、TNF-α、ET、iNOS及NO含量均显著升高,而SOD及IL-10含量下降。与模型对照组比较,荞麦黄酮各剂量组（100、200、400mg/kg）能不同程度改善上述指标。结论：荞麦黄酮在100~400 mg/kg剂量范围内对脑缺血再灌注损伤大鼠具有一定保护作用,其机制可能与抗氧化、抗炎及降低血管活性物质ET、NO含量相关。Objective： To investigate protective effect and mechanism of buckwheat flavones（ BF） cerebral ischemia reperfusion injury in rats and explore possible mechanisms. Methods： SD rats were randomly divided into Sham group,model group,BF groups with various dosages（ 100,200 and 400 mg /kg） and nimodipine group（ 6 mg /kg）. The rats were given drugs by ig administration once per day,for 5 d. At 2 h after last administration,the model of cerebral ischemia in rats was made by middle cerebral artery occlusion-reperfusion. At 24h after perfusion,the behavior,cerebral infarct size and water content of cerebrum in rats were determined,and SOD,MDA,IL-1β,IL-10,TNF-α,ET,iNOS and NO levels in serum were tested. Results： Compared with sham group,values of nerve behavior,cerebral infarct size,water content of cerebrum,MDA,IL-1β,TNF-α,ET,iNOS and NO significantly increase,while levels of SOD and IL-10 decrease（ P 〈 0. 01）. Compared with model group,BF with various doses（ 100,200,400 mg /kg） could improve above each index（ P 〈 0. 05,P 〈 0. 01）. Conclusion： BF in the dose of 100 ~ 400 mg /kg has protective effect on cerebral ischemia reperfusion injury in rats,its possible mechanisms are in part associated with antioxygenation and anti-inflammatory action and decrease the levels of vascular active substances such as ET,NO.

关 键 词：荞麦黄酮 脑缺血再灌注损伤 

分 类 号：R285[医药卫生—中药学]