黄芪总苷对脾虚大鼠胃粘膜TFF1和EGF表达的影响  

Effect of astragnaloside on the TFF1 and EGF in gastric mucosa with the spleen deficiency rats

作　　者：米红[1] 李燕舞[2] 王晓燕[3] 巫燕莉[2] 王汝俊[2] 刘凤斌[3] 

机构地区：[1]广州中医药大学 [2]广州中医药大学脾胃研究所 [3]广州中医药大学第一附属医院,广州510405

出　　处：《中药药理与临床》2014年第3期75-78,共4页Pharmacology and Clinics of Chinese Materia Medica

基　　金：广东省中医药管理局课题(No:20121221)

摘　　要：目的:探讨黄芪总苷对冷水-束缚应激状态下脾虚大鼠胃粘膜TFF1、EGF mRNA和蛋白表达的影响。方法:大鼠70只分为7组,即正常对照组、脾虚组、模型(脾虚应激)组、黄芪总苷(高剂量200mg/kg、中剂量100mg/kg、低剂量50mg/kg)组、补中益气丸3.7g/kg组。采用大黄致脾虚法复制脾虚模型大鼠,水浸-束缚法给予脾虚大鼠应激,酶联免疫(ELISA)法检测TFF1、EGF蛋白表达变化,RT-PCR法测定TFF1、EGF的mRNA表达变化。结果:与正常组比较,脾虚组大鼠胃粘膜TFF1 mRNA和蛋白表达下降,EGF mRNA低表达,而脾虚应激组TFF1、EGF mRNA和蛋白表达增加;与脾虚应激组相比,补中益气丸组和黄芪总苷(200mg/kg)组TFF1和EGF表达均明显增加。结论:黄芪总苷对脾虚大鼠胃粘膜的保护作用机制可能与影响TFF1和EGF有关。Objective： To investigate the effects of astragaloside（ AST） on the TFF1 and EGF in gastric mucosa of spleen deficiency rats under stress. Methods： 70 SD rats were selected and randomly divided into 7 groups： the control group,the spleen deficiency group,the model group,the AST（ high dose-200mg /kg,middle dose-100mg /kg,and low dose-50 mg /kg） group and the Buzhongyiqi pill group. The rhubarb decoction was used to establish spleen deficiency model,then water immersion and restraint method was used to establish the stress model.The protein express of the TFF1 and EGF were measured by ELISA; and the mRNA expression of the TFF1 and EGF were detected by RTPCR. Results： Comparing with the control group,the protein and mRNA expression of TFF1 were decreased in the deficiency group（ P 〈 0.05）,and the mRNA expression of EGF were lower（ P 〈 0. 05）; while the protein and mRNA expression of TFF1 in the model group were decreased（ P 〈 0. 05）,the mRNA expression of the EGF were increased（ P 〈 0. 05）. Comparing with the model group,the mRNA expression of the TFF1,EGF in the AST（ high） group and the Buzhongyiqi pill group were increased（ P 〈 0. 05）. Conclusion： One of the protection mechanisms of AST on gastric mucosal is perhaps concerned with the activity TFF1 and EGF.

关 键 词：黄芪总苷 三叶因子 表皮生长因子 

分 类 号：R285[医药卫生—中药学]