白术多糖对神经细胞毒性及抗缺氧诱导的神经细胞凋亡的影响  

Study of Atractylodes macrocephalaon polysaccharides on the neuron toxicity and anti-apoptosis induced by hypoxia /reoxygenation

作　　者：胡微煦[1] 向勤[2] 付爱珍 文珠 何丹[5] 胡国柱[5] 

机构地区：[1]复旦大学附属中山医院肿瘤放疗科,上海200032 [2]南昌大学研究生院医学部,南昌330006 [3]樟树市人民医院,樟树331200 [4]江西省医学科学研究院,南昌330006 [5]江西省人民医院,南昌330006

出　　处：《中药药理与临床》2014年第3期89-91,共3页Pharmacology and Clinics of Chinese Materia Medica

基　　金：江西省科技厅社会发展计划(2007BS22602)

摘　　要：目的:探讨白术多糖的毒性及抗神经细胞缺氧性凋亡作用。方法:研究分为正常对照组、凋亡阳性组、白术多糖0.025g/L组、白术多糖0.05g/L组、白术多糖0.1g/L组、白术多糖0.25g/L组。采用"Neurobasal+B27"体外神经细胞无血清培养,免疫细胞化学鉴定神经细胞,MTT测定药物毒性,AnnexinV/PI荧光双染流式细胞仪及Hochest33342荧光染色检测细胞凋亡。结果:白术多糖在1.0g/L之内预处理神经细胞对其无毒性作用。白术多糖在0.025g/L^0.05g/L范围显著地改善缺氧对神经细胞生长的抑制,在0.025g/L^0.25g/L范围内显著地抑制缺氧复氧诱导的神经细胞早期凋亡。结论:白术多糖在一定浓度范围内具有抗缺氧复氧诱导的细胞凋亡作用。Objective： To explore the effect of Atractylodes macrocephalaon polysaccharides（ AMPS） on cytotoxicity and preventing the neuron apoptosis indued by hypoxia. Methods： The study was divided into normal control group（ group C）,apoptosis positive group induced by hypoxia（ group A）,experiment 1 group treated by 0. 025g /L AMPS（ AMPS1）,experiment 2 group treated by 0. 05g /L AMPS group（ AMPS2）,experiment3 group treated by0.1g/L AMPS group（ AMPS3）,experiment4 group treated by0.25 g/L AMPS group（ AMPS4）.Neurons were cultured by Neurobasal ＋ B27 in serum-free in vitro. Neurons were identified by immunocytochemistry. Drug toxicity was determined by MTT assay,Neuron apoptosis was examined by Annexin V- FITC /PI in flow cytometry and Hoechst 33342 fluorescent staining under fluorescence microscope. Results： The AMPS was non-toxic for neurons pretreated within 1. 0 g /L. The AMPS significantly prevented the inhibition of neuron growth from hypoxia from 0. 025g /L to 0. 05g /L,（ P 〈 0. 05）. Early apoptosis of neurons induced by hypoxia-reoxygenation was significantly inhibited by AMPS from 0. 025g /L to 0. 25g /L（ P 〈 0. 05）. Conclusion： The AMPS has an effect on inhibitting early apoptosis of neurons induced by hypoxia-reoxygenation in a certain concentration range.

关 键 词：白术多糖 神经细胞 毒性 缺氧 凋亡 

分 类 号：R285[医药卫生—中药学]