Cytocompatibility Evaluation of Grafted IKVAV PLEOF Hydrogels with Bone Marrow Mesenchymal Stem Cells  

作　　者：李宾斌 ZHANG Ping YIN Yixia QIU Tong TAO Yuan WANG Xinyu 李世普 

机构地区：[1]State Key Laboratory of Advanced Technology for Materials Synthesis and Processing, and Biomaterials Science and Engineering Research Center, Wuhan University of Technology

出　　处：《Journal of Wuhan University of Technology(Materials Science)》2014年第4期824-831,共8页武汉理工大学学报（材料科学英文版）

基　　金：Funded by theNational Basic Research Program of China(No.2011CB606205);the Self-determined and Innovative Research Funds of WUT(No.2012-YB-007)

摘　　要：The novel hydrogels-grafted IKVAV poly（lactide-co-ethylene oxide-co-fumarate）（PLEOF） hydrogels（GIPHs） were developed. The rat bone marrow mesenchymal stem cells（BMMSCs） were employed, and the cell vitality and apoptosis assays were carried out to evaluate the cytocomptibility of GIPHs. Our data demonstrated that the infl uence of GIPHs on the proliferation of BMMSCs was in a concentration and time dependent manner. The proliferative ability of BMMSCs in GIPHs-treated group（100 μg/mL） after 72 h presented a maximum response which was 30.1% more than that of control group. The numbers of apoptotic cells in GIPHs-treated group（100 μg/mL） were just as much as that of control group after 24 h treatment. The GIPHs are able to provide an appropriate environment for BMMSCs survival and proliferation.The novel hydrogels-grafted IKVAV poly（lactide-co-ethylene oxide-co-fumarate）（PLEOF） hydrogels（GIPHs） were developed. The rat bone marrow mesenchymal stem cells（BMMSCs） were employed, and the cell vitality and apoptosis assays were carried out to evaluate the cytocomptibility of GIPHs. Our data demonstrated that the infl uence of GIPHs on the proliferation of BMMSCs was in a concentration and time dependent manner. The proliferative ability of BMMSCs in GIPHs-treated group（100 μg/mL） after 72 h presented a maximum response which was 30.1% more than that of control group. The numbers of apoptotic cells in GIPHs-treated group（100 μg/mL） were just as much as that of control group after 24 h treatment. The GIPHs are able to provide an appropriate environment for BMMSCs survival and proliferation.

关 键 词：proliferation IKVAV hydrogels vitality apoptosis 

分 类 号：TQ427.26[化学工程]