In vitro Characterization of PBLG-g-HA/PLLA Nanocomposite Scaffolds  

作　　者：廖岚 YANG Shuang Richard J Miron WEI Junchao ZHANG Yufeng 张萌 

机构地区：[1]School of Materials Science and Engineering, Nanchang University [2]School of Stomatology, Nanchang University [3]The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) & Key Laboratory of Oral Biomedicine Ministry of Education, School & Hospital of Stomatology, Wuhan University [4]Department of Oral Implantology, School of Stomatology, Wuhan University

出　　处：《Journal of Wuhan University of Technology(Materials Science)》2014年第4期841-847,共7页武汉理工大学学报（材料科学英文版）

基　　金：Funded by the National Natural Science Foundation of China(81271108&51203073);the Project from Education Department of Jiangxi Province(GJJ13107)

摘　　要：The purpose of the present study was to synthesize a new composites scaffold containing poly（γ-benzyl-L-glutamate） modified hydroxyapatite/（poly（L-lactic acid））（PBLG-g-HA/PLLA） and to investigate their in vitro behaviour on bone mesenchymal stromal cells（BMSCs）. The results demonstrated that BMSC proliferation was signifi cantly increased on PBLG-g-HA/PLLA scaffolds after 3 and 7 days post seeding when compared to PLLA and HA/PLLA scaffolds. The in vitro osteogenic differentiation also favoured the composite PBLG-g-HA/PLLA scaffolds when compared to controls by signifi cantly increasing Runx2, ALP or osteocalcin mRNA expression as assessed by real-time PCR. The results illustrate the potential of PBLG-g-HA/PLLA scaffolds for bone tissue engineering applications. And the in vivo testing further confi rms the PBLG-gHA/PLLA scaffolds＇ potentioal for healing critical bone defects.The purpose of the present study was to synthesize a new composites scaffold containing poly（γ-benzyl-L-glutamate） modified hydroxyapatite/（poly（L-lactic acid））（PBLG-g-HA/PLLA） and to investigate their in vitro behaviour on bone mesenchymal stromal cells（BMSCs）. The results demonstrated that BMSC proliferation was signifi cantly increased on PBLG-g-HA/PLLA scaffolds after 3 and 7 days post seeding when compared to PLLA and HA/PLLA scaffolds. The in vitro osteogenic differentiation also favoured the composite PBLG-g-HA/PLLA scaffolds when compared to controls by signifi cantly increasing Runx2, ALP or osteocalcin mRNA expression as assessed by real-time PCR. The results illustrate the potential of PBLG-g-HA/PLLA scaffolds for bone tissue engineering applications. And the in vivo testing further confi rms the PBLG-gHA/PLLA scaffolds＇ potentioal for healing critical bone defects.

关 键 词：hydroxyapatite poly（L-lactide） tissue engineering biomaterials 

分 类 号：R318.08[医药卫生—生物医学工程] TB332[医药卫生—基础医学]