A Novel Fluorescence Probe 9-(4-(1,2-diamine)benzeneN^1-phenyl)acridine for Nitric Oxide Determination  

作　　者：丁莉芸 袁芳 HUANG Lanfen HUANG Jun LIU Xiaofang 梁兵 

机构地区：[1]National Engineering Laboratory for Fiber Optic Sensing Technology, Wuhan University of Technology [2]Department of Health, Qilu Hospital, Shandong University [3]Key Laboratory of Neurogenetics and Channelopathies of Guangdong Province and the Ministry of Education of China, Institute of Neuroscience and the Second Affiliated Hospital of Guangzhou Medical University [4]School of Chemical Engineering, Wuhan University of Technology [5]Department of Neurology, Qilu Hospital, Shandong University

出　　处：《Journal of Wuhan University of Technology(Materials Science)》2014年第4期848-853,共6页武汉理工大学学报（材料科学英文版）

基　　金：Funded by the National Natural Science Foundation of China(Nos.50802069,81100890,51272191);the Fundamental Research Funds for the Central Unversities(WUT:2013-IV-010);the Students Innovation and Entrepreneurship Training Program of WHUT(20141049701012)

摘　　要：A novel fluorescent probe 9-（4-（1,2-diamine）benzene-N1-phenyl）acridine（DABPA） was synthesized for the detection of nitric oxide（NO） and characterized by IR, 1H-NMR and EI-MS spectroscopy. Based on a photoelectron transfer mechanism, the fl uorescence intensities of DABPA were investigated with the different concentrations of NO. Under the optimal experimental conditions, the fl uorescence intensity of DABPA had a good linear relationship（R2=0.9977） with NO concentration in the range from 1×10-7 to 1.5×10-6 mol/L with a detection limit of 1×10-8 mol/L. The cytotoxicity induced by DABPA was evaluated by the MTT（3-（4,5-dimethylthiazol-2-yl）-2,5diphenyl tetrazolium bromide） assay for biological application. Furthermore, the probe DABPA had also been successfully applied to real-time image NO produced in PC12 cells in the presence of L-arginine.A novel fluorescent probe 9-（4-（1,2-diamine）benzene-N1-phenyl）acridine（DABPA） was synthesized for the detection of nitric oxide（NO） and characterized by IR, 1H-NMR and EI-MS spectroscopy. Based on a photoelectron transfer mechanism, the fl uorescence intensities of DABPA were investigated with the different concentrations of NO. Under the optimal experimental conditions, the fl uorescence intensity of DABPA had a good linear relationship（R2=0.9977） with NO concentration in the range from 1×10-7 to 1.5×10-6 mol/L with a detection limit of 1×10-8 mol/L. The cytotoxicity induced by DABPA was evaluated by the MTT（3-（4,5-dimethylthiazol-2-yl）-2,5diphenyl tetrazolium bromide） assay for biological application. Furthermore, the probe DABPA had also been successfully applied to real-time image NO produced in PC12 cells in the presence of L-arginine.

关 键 词：nitric oxide fluorescent probe 9-（4-（1 2-diamine）benzene-N1-phenyl）acridine 

分 类 号：O626[理学—有机化学] O657.3[理学—化学]