血清胱抑素C在模拟高强度一次性力竭军事训练大鼠早期肾损伤诊断中的作用  

Significance of serum cystatin C on early detection of acute kidney injury caused by high intensity military training

作　　者：周春华[1] 陈洪 李斌[1] 张朝阳[1] 白晓龙[1] 李洪艳[1] 

机构地区：[1]北京海军总医院肾内科,100048 [2]北京海军总医院干部保健科,100048

出　　处：《解放军医学杂志》2014年第8期660-663,共4页Medical Journal of Chinese People's Liberation Army

摘　　要：目的探讨血清胱抑素C(Cys C)在大鼠模拟高强度一次性力竭军事训练肾损伤模型中的早期诊断效能,并与传统诊断标志物进行比较。方法 40只雄性12周龄SD大鼠随机均分5组(n=8):对照组(常规喂养,不加干预,置大鼠代谢笼留24h尿液后颈椎脱臼处死)及训练后即刻组(0h)、6h组、24h组、48h组。训练组采用高温、高湿环境下力竭负重跑台运动构建高强度一次性力竭军训肾损伤模型,按训练前、训练后0、6、24、48h检测血清Cys C、血肌酐(SCr)、尿素氮(BUN)、内生肌酐清除率(CCr)、肾小管半定量积分。结果训练后0、6、24h大鼠血清Cys C、BUN、SCr与对照组比较明显升高,0h组为峰值,48h恢复至对照组水平。Cys C与BUN、Cr呈显著正相关,与CCr呈显著负相关。受试者工作曲线(ROC)分析显示,训练后各时间点曲线下面积值(AUC)Cys C均高于BUN、Cr。结论高强度一次性力竭军训后即刻血清Cys C即升高,与BUN、Cr呈正相关,与CCr呈负相关,诊断肾损伤效能优于Cr、BUN。Objective To investigate the significance of determination of serum cystatin C （Cys C） on the early diagnosis of acute kidney injury in a rat model induced by mimetic high intensity military training, and compare its diagnostic efficacy with that of traditional markers. Methods Forty male SD rats, 12 weeks old, were randomly divided into 5 groups （8 each）. Rats in control group were fed a standard diet, and housed in metabolic cages to collect 24-hour urine, and they were then sacrificed by cervical dislocation. Rats in training group were subdivided into 0, 6, 24, 48h groups after mimetic high intensity training. Animals in training groups were employed to reproduce the animal model of acute kidney injury by one-period high intensity training, i.e. forcing the animals running on a treadmill under conditions of high temperature, high humidity and weight-bearing. The serum concentrations of Cys C, serum creatinine （SCr）, blood urea nitrogen （BUN）, creatinine clearance （CCr） and the renal tubular semi-quantitative score were determined before and 0, 6, 24 and 48h after training. Results The serum levels of Cys C, BUN and SCr were significantly elevated in 0, 6 and 24h post-training groups, with the peak increase in Oh group, and they were higher than those in control group （P〈0.05）. Then the levels declined to baseline on 48h post-training. The serum level of Cys C was positively correlated with that of SCr and BUN significantly, while negatively correlated with that of CCr. ROC analysis showed the area under curve （AUC） of Cys C was higher than those of SCr and BUN at each time after training. Conclusions The serum level of Cys C increases immediately after an exhausting training, and it is positively correlated with SCr and BUN significantly, but negatively correlated with CCr. The diagnostic efficacy ofCys C is better than that of SCr or BUN.

关 键 词：肾损伤 军事训练 胱抑素C 模型 动物 

分 类 号：R692[医药卫生—泌尿科学]