9.4T磁共振弥散张量纤维束成像对SD大鼠C6胶质瘤模型肿瘤生长特点随时间变化的影像学分析  

Application of 9.4 T of DTT in the diagnosis of cerebral gliomas of SD rat

作　　者：马璐[1] 余永强[1] 

机构地区：[1]安徽医科大学第四附属医院放射科,合肥230022

出　　处：《安徽医科大学学报》2014年第9期1233-1237,共5页Acta Universitatis Medicinalis Anhui

基　　金：安徽省自然科学基金(编号:99044530);安徽省教育厅自然科学基金(编号:001kj137)

摘　　要：目的探讨9.4 T磁共振弥散张量纤维束成像(DTT)对SD大鼠C6胶质瘤模型皮质纤维束(CST)随着肿瘤发展所致的一系列影像学变化,并通过相关病理检验验证影像诊断中的意义。方法 1使用立体定向法对20只成年雄性的SD大鼠的右脑尾状核注入体积为10μl含106个的C6胶质瘤细胞悬浮液。2运用9.4 T行常规磁共振(MRI)、弥散张量成像(DTI)和DTT检查后,运用Function tool软件处理,得到各向异性分数图[纤维束重组成像(FA)]图和方向编码彩色图及双侧CST 3D纤维束重建图对肿瘤病灶区域及肿瘤周边水肿区及正常白质区进行FA值及平均弥散系数(MD)值测量,进行FA图像信号强度及对比度的分析,并且观察分析不同时期的SD大鼠C6脑胶质瘤对皮质脊髓束解剖关系的影响,然后行HE染色进行病理证实。结果所有SD大鼠C6脑胶质瘤模型均完成3D纤维束FA图像重建。测量的FA值在3个区域比较差异(肿瘤实质区、周围水肿带、正常脑白质区)均有统计学意义(P<0.05),水肿带和肿瘤实质区测量的FA值差异无统计学意义(P>0.05),而3个部位的测量的MD值差异有统计学意义(P<0.05),DTT显示随着时间的推移,脊髓纤维束容易受到脑肿瘤占位、浸润、瘤周水肿带等的影响,纤维束可出现破坏和移位。结论 9.4 T磁共振DTT能对不同时期SD大鼠C6胶质瘤模型做出直观观察,能较好显示皮质纤维束解剖关系的改变,病理上的改变也证实影像上的分析。Objective To investigate the 9.4 T of DTF in the diagnosis of SD rat C6 cerebral gliomas model about the change of CST on tumor progression and pathology confirmed. Methods ① 10 txl C6 cell suspension of 106 cells number were implanted into the right caudate nucleus of brain hemisphere of 20 adult male SD rat with ster- eotactic technique. ② Application of 9.4 T of DTT and DTI, meanwhile the routine T1-weighted imaging（ T1 W1）, T2-weighted imaging（ T2W1）, contrast-enhanced T1W1, FLAIR imaging and diffusion tensor of the brain were ac- quired. Anatomic relationship between cerebral glioma and surrounding white matter fiber tracts was analyzed and measured the values of FA and MD in different areas on fractional anisotropic （FA） map, color-coded directional map, three-dimensional （3D） white matter fiber tracts map, then stained with routine HE. Results All of SD rat C6 cerebral gliomas models 3D white matter fiber tracts map by means of FA maps of DT1 were successfully comple- ted. Apparently significant differences of FA values were found in solid tumor, surrounding edema, compared with normal white matter regions （P 〈 0. 05 ） ;but there was no significant difference among solid tumor and surrounding edema region （P 〉 0. 05 ） ; there were significant differences of MD values between solid tumor, surrounding edema and normal white matter region （P 〈 0. 05 ）. Conclusion The 9.4 T of DTT offers the optimal visualization of An- atomic relationship between cerebral glioma and surrounding white matter fiber tracts in the different periods of the tumor moder.

关 键 词：9.4T磁共振 DTT DTI MRI C6胶质瘤模型 SD大鼠 FA值 

分 类 号：R338[医药卫生—人体生理学] R739.41[医药卫生—基础医学]