辛二酰苯胺异羟肟酸对2型糖尿病阻碍舒芬太尼后处理心肌保护效应的影响  

Suberoylanilide hydroxamic acid in the attenuation of cardioprotective effect by sufentanil postconditioning in type 2 diabetes mellitus rats

作　　者：陈菁菁[1] 顾尔伟[1] 鲁显福[1] 张雷[1] 刘训芹[1] 程岑[1] 

机构地区：[1]安徽医科大学第一附属医院麻醉科,合肥230022

出　　处：《安徽医科大学学报》2014年第9期1279-1283,共5页Acta Universitatis Medicinalis Anhui

基　　金：国家自然科学基金专项主任基金研究项目(编号:81341014)

摘　　要：目的探讨组蛋白去乙酰酶抑制剂辛二酰苯胺异羟肟酸(SAHA)对2型糖尿病(T2DM)阻碍舒芬太尼后处理心肌保护效应的影响。方法应用SPF级雄性SD大鼠建立糖尿病(DM)和非糖尿病(NDM)模型,各自随机分为6组:假手术组(S组)、缺血再灌注组(I/R组)、舒芬太尼后处理组(SP组)、SAHA加舒芬太尼后处理组(SASP组)、二甲基亚砜加舒芬太尼后处理组(DSSP组)以及SAHA组(SA组)。测定血浆心肌肌钙蛋白I(cTnI)浓度并计算梗死区和缺血危险区之比(IS/AAR)。结果与NDM-I/R组相比,NDM-SP、NDM-SASP以及NDM-DSSP组的IS/AAR以及血浆cTnI浓度均下降(P<0.05);与DM-I/R组比较,DM-SP组、DM-DSSP组及DM-SA组IS/AAR和血浆cTnI差异无统计学意义(P>0.05),但DM-SASP组IS/AAR以及血浆cTnI浓度下降(P<0.05)。结论 SAHA可以逆转被T2DM阻碍的舒芬太尼后处理心肌保护效应,推测T2DM阻碍舒芬太尼后处理心肌保护的机制可能与组蛋白乙酰化/去乙酰化有关。Objective To investigate the effects of suberoylanilide hydroxamie acid （SAHA, as a histone deacety- lase inhibitor） in the attenuation of cardioprotective effect by sufentanil postconditioning in type 2 diabetes mellitus （T2DM） rats. Methods Male SD rats of SPF grade were included to establish diabetic as well as non-diabetic rat models. Both types were randomly divided into 6 groups respectively： sham-operated group （Group S） ; ischemia- reperfusion group （Group I/R） ; sufentanil postconditioning group （Group SP） ; SAHA plus sufentanil postcondi-tioning group （Group SASP） ; DMSO plus sufentanil postconditioning group （Group DSSP） ; SAHA group （Group SA）. Both measurement of plasma concentration of cardiac troponin I （cTnI） and calculation of IS/AAR were ac- complished. Results In group NDM-SP, NDM-SASP and NDM-DSSP, the IS/ARR and plasma concentrations of cTnI significantly decreased compared with group NDM-I/R （P 〈 0. 05 ）. There was no statistical significance a- mong groups of DM-I/R, DM-SP, DM-DSSP and DM-SA in the IS/ARR and plasma concentrations of cTnI （P 〉 0.05 ）, while adding the intervention of sufentanil postconditioning to group DM-SP brought down indexes men- tioned above dramatically （P 〈 O. 05 ）. Conclusion In the case that SAHA could restore the cardioprotective effects of sufentanil postconditioning which were once blocked due to T＇2DM, there might exist links between the mechanism of this obstruction and modification of histone acetylation/deacetylation.

关 键 词：辛二酰苯胺异羟肟酸 2型糖尿病 舒芬太尼 

分 类 号：R979.9[医药卫生—药品] R587.1[医药卫生—药学]