长座虫草子实体和菌丝体中碱基和核苷类物质的HPLC分析  

Determination of thirteen nucleosides and nucleobases in the fruiting body and mycelium of Ophiocordyceps longissima by HPLC

作　　者：张勋[1] 程文明[1,2] 李春如[3] 吴昆[1] 陆维丽[1] 李俊[1] 

机构地区：[1]安徽医科大学药学院、安徽天然药物活性研究省级重点实验室、安徽省天然药物活性成分工程技术研究中心,合肥230032 [2]天然药物活性组分与功效国家重点实验室中国药科大学,南京210009 [3]安徽农业大学林学院、安徽省微生物防治重点实验室,合肥230036

出　　处：《安徽医科大学学报》2014年第9期1283-1286,共4页Acta Universitatis Medicinalis Anhui

基　　金：国家自然科学基金(编号:30570004);天然药物活性组分与功效国家重点实验室资助项目(编号:SKLNMKF201416);安徽省自然科学基金(编号:090413105)

摘　　要：建立高效液相色谱法同时测定长座虫草中13种核苷和碱基类物质，色谱条件为：TSKgelODS-100V色谱柱（250mm×4．6mm，5斗m），以甲醇-0．Olmol／LKH2P04水溶液系统为流动相，梯度洗脱，流速1ml／min，检测波长260nm，柱温20℃。13种成分在50min内均达到基线分离，线性关系良好（r≥0．9990，n=8）；平均回收率在98．4％～107．5％（RSD〈2．0％，n=5）。结果表明长座虫草子实体中胞嘧啶、次黄嘌呤含量相对较高；菌丝体中尿嘧啶、鸟苷、腺苷含量较高，尿苷在菌丝体和子实体中含量均较高。Abstract An HPLC method was established for simultaneous determination of 13 nucleosides and nucleobases in Ophiocordyceps longissima. The separation was performed on a TSK gel ODS-100V column （250 mm x4. 6 mm,5 μm） using gradient elution with an aqueous potassium dihydrogen phosphate-methanol solution at 260 nm. The flow rate was 1.0 ml/min and column temperature was 20 ~C. Thirteen nucleosides and nucleobases were analyzed with- in 50 min. The calibration curves showed good linear relationship （r≥0. 999 0, n = 8 ） and the method exhibited excellent accuracy with a recovery of 98.4 to 107.5 % （ RSD 〈 2.0%, n = 5 ）. Higher content of cytosine and hy- poxanthine were detected in the fruiting body. The contents of adenosine, uracil, guanosine were relatively rich in mycelium of Ophiocordyceps longissima. Uridine was abundant both in fruiting body and mycelium of Ophiocordyceps longissima.

关 键 词：长座虫草 高效液相色谱 核苷 碱基 提取 含量测定 

分 类 号：R284.1[医药卫生—中药学] R931.6[医药卫生—中医学]