小麦CPP转录因子基因的电子克隆及生物信息学分析  

Electronic Clone and Bioinformatics Analysis of CPP Transcription Factor Genes from Wheat

作　　者：孟超敏[1] 姬俊华[2] 李雪林[1] 尚家 

机构地区：[1]河南科技大学农学院,河南洛阳471003 [2]洛阳理工学院环境工程与化学系,河南洛阳471023

出　　处：《生物技术》2014年第4期39-42,共4页Biotechnology

基　　金：河南科技大学人才基金项目("作物抗逆相关转录因子家族新基因的筛选与功能鉴定";No.09001407)资助

摘　　要：目的:运用电子克隆的方法获得小麦中的CPP转录因子基因。方法:以水稻的CPP转录因子作为探针,对小麦的EST数据库进行搜索,应用相关软件进行聚类分析、拼接组装和延长。结果:克隆出4个小麦CPP基因,分别命名为TaCPP1、TaCPP2、TaCPP3和TaCPP4,序列长度分别为977bp、3 022bp、1 582bp和1 156bp,开放阅读框为975bp、2 298bp、720bp和750bp。4个CPP类型蛋白质都具有一个或两个CXC域,而且多数还拥有完整的CRC结构域。结论:4个小麦CPP基因与水稻CPP蛋白具有高度的同源性。研究结果为进一步实验克隆和研究其功能提供了理论基础。Objeclive：CPP genes from wheat were cloned in silico cloning. Method：4 CPP genes were cloned by blasting the EST database and using the bioinformatics soft with the rice CPP as a querying probe. Result：In this study,bioinformatics methods based on EST identi- fied 4 CPP genes in wheat which were named TaCPP1, TaCPP2, TaCPP3 and TaCPP4 respectively. The cDNA length of 4 wheat CPP genes were 977bp,3 022bp,1 582bp and 1 156bp,whieh included 975bp,2 298bp,720bp and 750bp open reading frame（ORF） respec- tively. Finally,the alignment of amino acid sequences suggested that every CPP- like protein had one or two CXC domains and most also had a complete CRC domain. Condusion：The phylogenetic tree suggested that the CPP transcription factors in wheat were near in relation- ship with the ones in rice, which provided the basis for predicting their fimetions. These results provided theory basis for the gene cloning by PCR technique and functional analysis.

关 键 词：小麦 CPP基因 电子克隆 生物信息学 

分 类 号：Q943.2[生物学—植物学] Q811.4