TRPV1受体基因敲除雌性小鼠背根神经节P2X3受体表达升高  

Upregulation of P2X3 receptors in dorsal root ganglion of TRPV1 knockout female mice

作　　者：方潇[1] 时霄寒 黄丽斌[1] 戎伟芳[2] 马蓓[1] 

机构地区：[1]中国人民解放军第二军医大学生理学教研室,上海200433 [2]上海交通大学医学院生理学教研室,上海200025

出　　处：《生理学报》2014年第4期431-437,共7页Acta Physiologica Sinica

基　　金：supported by the Project of Undergraduate Students Creative Fund of the Secondary Military Medical University;China(No.ZD2012030)

摘　　要：本文旨在考察瞬时受体电位香草酸亚型1(transient receptor potential vanilloid 1,TRPV1受体)基因敲除后小鼠慢性炎症条件下机械痛阈的改变。通过足底注射给予完全弗氏佐剂(20μL)介导雌性小鼠慢性炎症痛的形成,利用弗莱毛测痛法测量TRPV1受体基因敲除型及野生型雌性小鼠在给药前1天和给药后8天内的机械痛阈。给药后第9天处死小鼠,利用蛋白质免疫印迹技术研究两组小鼠脊髓背根神经节(dorsal root ganglion,DRG)和脊髓背角中c-Fos蛋白和P2X3受体表达的差别。结果显示,与野生型小鼠相比,TRPV1受体基因敲除型小鼠基础机械痛阈明显增高(P<0.05);足底注射CFA后第3天起,TRPV1受体基因敲除型小鼠机械痛阈高于野生型小鼠(P<0.05);蛋白质免疫印迹结果表明TRPV1受体基因敲除型小鼠DRG和脊髓背角中c-Fos蛋白的表达明显低于野生型小鼠(P<0.01,P<0.05),TRPV1受体基因敲除型小鼠DRG中P2X3受体的表达明显高于野生型小鼠(P<0.05)。以上结果证明TRPV1受体可能通过调节DRG和背角中的c-Fos蛋白的表达以及影响P2X3受体在DRG中的表达从而影响外周机械痛阈。The study was aimed to investigate the changes in mechanical pain threshold in the condition of chronic inflammatory pain after transient receptor potential vanilloid 1 （TRPV1） gene was knockout. Hind-paw intraplantar injection of complete freund＇s adju- vant （CFA, 20 btL） produced peripheral inflammation in wild-type and TRPV1 knockout female mice. The mechanical pain thresholds were measured during the 8 days after injection and pre-injection by using Von-Frey hair. Nine days after injection, mice were killed and the differences of expression of c-Fos and P2X3 receptor in the dorsal root ganglia （DRG） and spinal cord dorsal horn were exam- ined by Western blotting between the two groups. Compared with that in wild-type mice, the mechanical pain threshold was increased significantly in TRPV1 knockout mice （P 〈 0.05）; 3 days after CFA injection, the baseline mechanical pain threshold in the TRPV1 knockout mice group was significantly higher than that in the wild-type mice group （P 〈 0.05）; The result of Western blotting showed that the expression of c-Fos protein both in DRG and spinal cord dorsal horn of TRPVI knockout mice group was decreased signifi- cantly compared with that in wild-type mice group （P 〈 0.01, P 〈 0.05）, while the expression of P2X3 receptor in DR（; of TRPV1 knockout mice group was increased significantly compared with that in wild-type mice group （P 〈 0.05）. Our findings indicate that TRPV1 may influence the peripheral mechanical pain threshold by mediating the expression of c-Fos protein both in DRG and spinal cord dorsal horn and changing the expression of P2X3 receptor in DRG.

关 键 词：TRPV1阳离子通道 P2X3受体 基因敲除小鼠 背根神经节 C-FOS蛋白 痛阈 

分 类 号：R402[医药卫生—临床医学]