不同程度间歇低氧对大鼠炎性细胞因子和脂肪因子的影响  

Effect of different degrees of intermittent hypoxia on inflammatory cytokines and adipokines in rats

作　　者：杨庆婵 朱慧 王彦 何庆 冯靖 陈宝元 

机构地区：[1]医科大学总医院呼吸科,天津300052 [2]医科大学总医院内分泌科,天津300052 [3]海河医院呼吸疾病研究所,天津市 300350

出　　处：《中华内分泌代谢杂志》2014年第8期693-696,共4页Chinese Journal of Endocrinology and Metabolism

基　　金：国家自然科学基金项目（81270144,30800507,81170071）

摘　　要：旨在测定不同程度间歇低氧暴露大鼠血清中炎性细胞因子和脂肪因子水平的变化。将160只成年雄性Wistar大鼠[体重（1804±15）g]分为5组，包括3个不同程度间歇低氧组（5％O2IH-1组；7．5％O2：IH-2组；10％O2：IH-3组）及持续低氧（CH）组（10％O2，CH组）和间歇正常氧对照组（IN组）。每组分别于暴露开始前及第2、4、6、8周后随机抽取8只大鼠，收集血清，采用葡萄糖氧化酶-过氧化物酶法测量血糖：采用双抗夹心酶联免疫吸附法（ELISA）测定其血清中胰岛素、肿瘤坏死因子（TNF）-α、白细胞介素（IL）-6、瘦素、脂联素和脂肪细胞核／质抽提物中NF—KB的光密度标化水平。结果显示IH-1、IH-2和IH-3组大鼠血清中血糖、胰岛素、TNF-α、IL-6、瘦素和脂肪细胞核NF—KB水平随暴露时间延长逐渐增加，且IH-1组明显高于IH-2和IH．3组（均P〈O．05），但TNF-α水平在第8周有下降趋势（P〈0．05）；脂联素水平随暴露时间延长逐渐下降。IH-1组明显低于IH-2和IH-3组（均P〈0．05）；细胞质NF-KB在各时间点及各组间无明显差异（均P〉O．05）。结果表明阻塞性睡眠呼吸暂停模式下间歇低氧可以激活NF-kB，导致大鼠系统性炎性细胞因子和脂肪因子分泌异常，且随着暴露程度增加，前炎症通道的激活，提示脂肪组织炎症程度不断加重。To study the effect of different levels intermittent hypoxia on inflammatory cytokines and adipokines in a rat model. 160 male Wistar rats were divided into five groups, three groups with intermittent hypoxia （5% O2 ： IH-1 group; 7.5% O2 ： IH-2 group; 10% O2 ： IH-3 group） , continuous hypoxia group （ 10% O2, CH group） ; and the intermittent normal oxygen control group （ IN）. Before the exposure and at the second, fourth, sixth, and eighth week after the exposure, eight rats in each group were selected randomly with blood samples collected. Blood glucose was measured by glucose oxidase-peroxidase （GOD-POD） reagents, double sandwich enzyme-linked immunosorbent assay （ ELISA ） was used to detect serum concentration of insulin, tumor necrosis factor alpha（ TNF）-α, interleukin （IL）-6, leptin, and adiponectin and adipocyte nuclear factor kappa B （NF-KB） levels. In IH-1, IH-2, and IH-3 group, blood glucose, insulin, TNF-α, IL-6, leptin, and nuclear NF-KB in rats showed consecutive increment after exposure to intermittent hypoxia, and those findings in IH-1 group were higher than those in IH-2 and IH-3 group （P〈0.05） , but the level of TNF-α dropped slightly on the 8th week. The level of adiponectin showed consecutive decrease from the outset and its level in IH-1 group was significantly lower than that in IH-2 and IH-3 group （ P〈0.05 ）. Cytoplasmic NF-KB levels did not have obvious difference in all these groups （ P〉 0.05 ）. These results demonstrate that intermittent hypoxia could activate NF-KB and result in extensive changes of proinflammatory cytokines and adipokines in the serum of rats. The release of pro-inflammatory cytokines and adipokines keeps pace with the degree of intermittent hypoxia.

关 键 词：间歇低氧 炎症 瘦素 脂联素 

分 类 号：R766[医药卫生—耳鼻咽喉科]