miR-130b在胶质瘤中表达及其对胶质瘤增殖与侵袭的影响  

Exepression of miR-130b in glioblastoma and its impact on proliferation and invasion of human glioma cells

作　　者：张瑞 徐修鹏 陈正新 董庆生 张雅旋 华领洋 王慧博 刘宁 

机构地区：[1]南京医科大学第一附属医院神经外科,江苏省210029

出　　处：《江苏医药》2014年第16期1864-1867,共4页Jiangsu Medical Journal

基　　金：国家自然科学基金(81201978;81172389);江苏省医学重点学科(XK201117)

摘　　要：目的探讨微小RNA 130b(miR-130b)在胶质瘤中表达及其对胶质瘤增殖与侵袭的影响。方法采用qRT-PCR技术检测miR-130b在人脑胶质瘤组织与正常脑组织中的表达。用antimiR-130b寡聚核苷酸转染细胞,通过CCK8细胞活性检测试剂盒、流式细胞术和Matrigel transwell实验检测miR-130b表达下调后对U87细胞增殖、周期和Matrigel transwell的影响。结果与正常脑组织比较,miR-130b在胶质瘤组织中高表达(P<0.05)。下调miR-130b抑制U87细胞增殖和侵袭(P<0.05)。结论 miR-130b在胶质瘤组织中高表达;下调其表达可以明显抑制胶质瘤细胞增殖与侵袭能力。Objective To explore the expression of microRNA-130b（miR-130b）in glioblastoma and its impact on proliferation and invasion of human glioma cells.Methods The expressions of miR-130 bwere detected using qRT-PCR in human glioma and normal brain tissues.The anti-miR-130 b oligonucleotide was transfected into glioma cells U87.CCK-8assay,flow cytometry and matrigel transwell assay were performed to investigate the impact of miR-130 bexpression on the proliferation and invasion of the glioma cells.Resluts Compared to the normal brain tissues,the expression of miR-130 bwas upregulated in glioma specimens.Down-regulation of miR-130 bin glioma cells could inhibit the proliferation of glioma cells,arrest these cells at G1 phase,and impede the invasion ability of these cells（P〈0.05）.Conclusion The expression level of miR-130 bis upregulated in glioma,which could remarkably inhibit the proliferation and invasion of the glioma cells.

关 键 词：胶质瘤 微小RNA-130b 

分 类 号：R651[医药卫生—外科学]