链状酰胺类化合物B07的体外杀精效果及其对精子质膜的影响  

Effect of B07 on Spermcidal Activity and Membrane Change of Human Sperm In Vitro

作　　者：职瑞娜 陆路[3] 戴秋云 王玉柱[2] 黄超[2] 夏敏杰[2] 丁训城[2] 李卫华[2] 姜世勃[3] 

机构地区：[1]复旦大学公共卫生学院,上海200032 [2]国家人口计生委计划生育药具重点实验室,上海市计划生育科学研究所,上海200032 [3]复旦大学上海医学院分子病毒学教育部/卫生部重点实验室,上海200032 [4]军事科学研究院生物工程研究所,北京100071

出　　处：《生殖与避孕》2014年第8期617-622,共6页Reproduction and Contraception

基　　金："十二五"国家科技支撑计划(2012BAI31B04);"十二五"国家科技重大专项(2012ZX10001007-009-003);"十二五"国家科技重大专项(子任务)(2013ZX10001006-003-002);上海市人口和计划生育委员会(2012JG03);国家自然科学基金青年基金项目(81100459)资助

摘　　要：目的：研究一种链状酰胺类化合物（B07）的体外杀精作用及其对精子质膜的影响。方法：上游收集高活力人类精子分别与0μg／ml（对照组），5-640μg／mlB07作用30min，随后用计算机辅助精液分析仪分析记录精子的活率及运动参数；用160μg／mlB07与精子作用，记录5min、30min、60min和120min时精子活动率：处理后的精子一部分经SYBR-14／PI双染后用荧光显微镜观察，另一部分通过电子显微镜来观察质膜的变化情况；并以壬苯醇醚科9）作为阳性对照。结果：随着B07浓度的升高，精子活率和活力呈下降趋势，最低有效浓度（MEC）为640gg／ml；荧光显微镜下经B07处理的精子呈橙色，而N9处理的精子呈红色；电子显微镜显示B07组精子头部肿胀起泡，尾部针孔样损伤，N9组精子呈溶解性改变。结论：B07具有体外杀精作用，且该作用有时间和浓度依赖性。且其对精子质膜的影响小于N9。Objective： To evaluate the spermicidal activity of B07 and its effect on spermatozoa membrane. Methods： Human spermatozoa after ＂swim-up＂ were incubated with two-fold serial dilutions of B07. The spermicidal activity of B07 was assessed by computer-assisted sperm analysis （CASA）. Sperms were stained and observed under fluorescence microscope. Scanning electron microscopy was used for topographical imaging of different membrane domains over the sperms. Results： Sperm motility and vitality descended with the increase of B07 concentration. The minimum effective concentration of B07 that induced immobilization of human sperm was 640 μg/ml. Fluorescence microscope examination showed that B07-treated sperm appeared orange while N9-treated red. The electron micrographs of B07-treated sperm showed slightly swollen membrane with some vesiculations and visibly perforations of sperm tail. Otherwise, N9-treated sperm were acutely damaged. Conclusion： The in vitro study proved the dose- and time-dependent spermicidal activity of B07. Furthermore, the membrane-damage effect orB07 is much less than that of N9.

关 键 词：链状酰胺类化合物B07 人类精子 杀精 质膜 

分 类 号：R965[医药卫生—药理学]