^(13)C同位素示踪法研究半纤维素与木素之间的连接方式  

Analysis of the Association between Hemicelluloses and Lignin by Carbon 13 Tracer Method

作　　者：石超群[1] 张婷婷[1] 谢益民[1,2] 王鹏[1,2] 梅秦源[1] 

机构地区：[1]湖北工业大学制浆造纸工程学院,湖北武汉430068 [2]湖北工业大学绿色轻工材料湖北省重点实验室,湖北武汉430068

出　　处：《造纸科学与技术》2014年第4期1-5,共5页Paper Science & Technology

基　　金：国家自然科学基金(31070521;31370574;31300494);湖北省教育厅青年人才项目(Q20131402)

摘　　要：为了阐明植物纤维原料中木素与半纤维素之间的相互关系,本文从甘露糖单元的角度分析糖与木素苯丙烷结构单元之间的连接方式。为了使生长中的植物的细胞壁甘露糖单元上的碳原子被碳-13同位素示踪并抑制甘露糖向木素转化,将带D-13C6-甘露糖、外源性木素前驱物松伯醇β-D-葡萄糖苷以及苯丙氨酸解氨酶的抑制剂一起投入到银杏植株中。通过碳-13丰度检测得知银杏中半纤维素被碳-13同位素成功标记,外源性的D-13C6-甘露糖主要在次生壁中发生聚合沉积。FT-IR谱图分析发现投入的外源性糖等没有影响银杏植株的正常生长,高分辨率CP/MAS13C-NMR证实了甘露糖单元的C6位置与木素苯丙烷结构上的α-C存在连接键,主要为醚键。In the present paper, chemical connections between carbohydrate and lignin were analyzed based on the perspectives of mannose to further understand the bonds between hemicelluloses and lignin in gymnosperms. D - 13C6 - Mannose and the inhibitor of phenylalanine ammonia - lyase was injected into a living ginkgo tree with exoge- nous lignin precursors to make the growing plant cell wall mannose units being marked by carbon - 13 and stop con- verting to lignin, respectively. The 13C abundance results show that the hemicelluloses was marked successfully, and deposition of hemicelluloses in the cell wall is effective in the secondary wall. FI - TR spectrum indicated that the metabolism of ginkgo was normally with exogenous injections. The high resolution CP/MAS 13C -NMR spectra confirmed the bounds, which mainly are ether linkage between C6 position of mannose units in hemicelllulose and α - C of lignin side.

关 键 词：木素-碳水化合物复合体 甘露糖 同位素示踪 碳13丰度 核磁共振 

分 类 号：TQ31[化学工程—高聚物工业]