两种提取蒙古黄芪miRNA方法的比较  

Compare with Two Protocols for microRNA Extraction from Astragalus membranaceus var. mongholicus

作　　者：吕帝瑾[1] 陈婧[1] 钟扬[1] 南蓬[1] 

机构地区：[1]复旦大学生命科学学院生物多样性与生态工程教育部重点实验室,上海200433

出　　处：《植物生理学报》2014年第8期1255-1258,共4页Plant Physiology Journal

基　　金：国家自然科学基金(81373963和30925004)

摘　　要：通过对两种miRNA提取方法——一步法和多步法进行比较研究,以期获得较高质量的蒙古黄芪不同器官的miRNA。实验结果表明,两种方法均可用于蒙古黄芪miRNA提取,二者存在着不同的优缺点,多步法提取成功率较高但步骤繁琐,相比之下一步法实验条件要求严苛但步骤简单、快捷省时,提取的蒙古黄芪miRNA完整性好,可以满足荧光定量PCR等进一步实验需要。In order to obtain better quality miRNA in different parts of Astragalus membranaceus var. mongholicus, two miRNA extraction methods （one-step method and multi-step method） were compared. The results showed that these two methods had different advantages and disadvantages, but both of them could be used for miRNA extraction. Multi-step method had higher success rate with cumbersome steps, one-step method needed strict experimental conditions but fast and time-saving, the miRNA isolated with one-step method had high integrity which could be used for quantitative RT-PCR and so on.

关 键 词：miRNA提取 蒙古黄芪 一步法 多步法 

分 类 号：S567.239[农业科学—中草药栽培]