电磁脉冲暴露对雄性BALB/c小鼠子代性别比的影响  

Effect of EMP on sex ratio of the offspring of male BALB/c mice

作　　者：李瑾慧 刘军叶[1] 蒋大鹏[1] 苗霞[1] 王亚峰[1] 郭奇彦 郭国祯[1] 

机构地区：[1]第四军医大学放射医学教研室,西安710032

出　　处：《中华放射医学与防护杂志》2014年第8期588-591,共4页Chinese Journal of Radiological Medicine and Protection

基　　金：国家重点基础研究发展计划（973计划）（2011CB503704）;国家自然科学基金（51277175,81072272）

摘　　要：目的 探讨雄性BALB/c小鼠电磁脉冲（electromagnetic pulse,EMP）暴露对子代性别比的影响.方法 BALB/c小鼠雄性33只,雌性66只.雄性小鼠按照体重随机分为10 000次、100 000次脉冲辐照组和假辐照组.雌性小鼠相同环境分隔饲养,用以合笼.辐照组给予场强35 kV/m,重复频率50 Hz,脉冲数分别为10 000次和100 000次的辐照,每日1次,连续辐照2周.辐照后按雌雄比2∶1合笼1周,1周后处死雄鼠,对比分析3组雄鼠的体重和睾丸重量,计算体睾指数;游离附睾中精子进行计数.孕鼠于孕20 d解剖取胎鼠组织进行PCR,鉴定子代性别比.结果 与假辐照组相比,10 000次辐照组和100 000次辐照组雄鼠的体重、睾丸重量和体睾指数差异不大;精子计数均有下降,但差异无统计学意义.10 000次辐照组子代性别比（雄性：雌性）为0.298明显低于假辐照组0.871（x2=9.37,P＜0.O1）;100 000次辐照组为0.826,与假辐照组相比差异无统计学意义.结论 雄性小鼠在场强35 kV/m,重复频率50 Hz,脉冲次数10 000次的EMP中暴露,可能导致子代雄性与雌性性别比降低.Objective To investigate the effects of EMP exposure to male BABL/c mice on the reproduction and its sex ratio.Methods Thirty-three male BALB/c mice were randomly divided into sham-exposed group,10 000 pulses/d exposed group,and 100 000 pulses/d exposed group with 50 Hz static magnetic field of 35 kV/m for 2 weeks consecutively,then the male mice were mated with unexposed females （1 male vs.2 female）.Mated males were sacrificed to examine the exposure effects on sperm number and testis index after 7 days of mating.The sex ratio of embryos collected from unexposed mated females was investigated by PCR.Results The sex ratio of offspring was 0.298 （14/47） in the 10 000 pulses/d exposed group,significantly lower than 0.871 （27/31） in the sham-exposed group.No statistical changes were observed in the sex ratio of offspring,sperm number,the weights of testis and testis index after 100 000 pulses/d exposure.Conclusions The exposure of male mice to 35 kV/m EMP at 10 000 pulses/d for 2 weeks consecutively may change the sex ratio of mice＇s offspring.

关 键 词：电磁脉冲 睾丸指数 精子计数 子代性别比 

分 类 号：R594.8[医药卫生—内科学]