针刺对戊四唑诱发惊厥大鼠海马葡萄糖调节蛋白78和C/EBP同源蛋白表达的影响  

Effects of Acupuncture Intervention on Expression of Glucose-regulated Protein 78 and C/EBP Homologous Protein in Hippocampal CA 1 Region in Rats with Hyperspasmia

作　　者：杨帆[1] 马允[2] 昂文平[2] 陈浩[1] 杜卫东[2] 吴生兵[3] 吕磊[4] 张道芹[4] 

机构地区：[1]安徽中医药大学针灸骨伤临床学院,合肥230038 [2]安徽中医药大学研究生部,合肥230038 [3]安徽中医药大学实验中心,合肥230038 [4]安徽中医药大学中西医结合临床学院,合肥230038

出　　处：《针刺研究》2014年第4期267-271,共5页Acupuncture Research

基　　金：国家自然科学基金(No.81072872)

摘　　要：目的:观察针刺对惊厥大鼠海马神经元内葡萄糖调节蛋白78(Grp 78)和C/EBP同源蛋白(CHOP)蛋白表达的影响,探讨针刺抗惊厥的可能机制。方法:SD大鼠随机分为正常对照组(n=6)、模型组(n=18)、针刺组(n=18)。腹腔注射戊四唑(50mg/kg)复制惊厥大鼠模型。针刺组立即针刺"百会""大椎"30min。各组分别于造模后2、12、48h采用免疫组化法观察各组大鼠海马CA 1区Grp 78和CHOP蛋白表达情况。结果:模型组大鼠在惊厥发作2、12h海马CA 1区Grp 78蛋白表达与正常组比较明显增强(P<0.01);针刺组大鼠在惊厥发作12h和48h与模型组比较Grp 78蛋白表达显著增加(P<0.01,P<0.05)。在惊厥发作各个时段与正常组比较,模型组大鼠海马CA 1区CHOP蛋白表达明显上调(P<0.01);针刺组海马CA 1区CHOP蛋白阳性表达在惊厥发作各个时段与模型组比较明显减少(P<0.05,P<0.01)。结论:针刺能明显调节惊厥大鼠海马神经元Grp 78蛋白和CHOP蛋白的表达,从而起到保护惊厥性脑损伤作用。Objective To observe the effect of acupuncture （Grp 78） and C/EBP homologous protein （CHOP） in the hippocampus ntervention on expression of glucose-regulated protein 78 n epilepsy rats so as to explore its mechanism underlying improvement of hyperspasmia-induced brain injury. Methods Forty-two SD rats were randomly divided into normal control group （n =6）, model group （n = 18）, and acupuncture group （n = 18）. The epileptic seizure model was established by intrape- ritonel injection of Pentylenetetrazol （50 mg/kg, 2 mL）. Manual acupuncture stimulation of ＂Baihui＂ （GV 20） and ＂Dazhui＂ （GV 14） was conducted for rats of the acupuncture group for 30 min. Two hours （h）, 12 h and 48 h after acupuncture intervention, the hippocampal tissue was sampled （6 rats at each time-point）. The expression levels of Grp 78 and CHOP proteins in the hippocampal CA 1 region were detected by immunohistochemistry. Results Compared with the normal group, the expression levels of Grp 78 protein at time-points of 2 h and 12 h, and those of CHOP protein at 2 h, 12 h and 48 h after epilpeptic seizure were significantly increased in the model group （ P〈0.01 ）. After acupuncture treatment, the expression levels of Grp 78 at 12 and 48 h were significantly increased, and those of CHOP protein at 2 h, 12 h and 24 h in the acupuncture group were considerably downregulated （P〈0.05, P^0.01）. Conclusion Acupuncture treatment can up-regulate Grp 78 protein expression and down-regulate CHOP protein expression level in epilepsy rats , which may contribute to its protective effect on seizure-induced brain injury.

关 键 词：惊厥 针刺 海马CA1区 葡萄糖调节蛋白78 C EBP同源蛋白 脑损伤 

分 类 号：R245.311[医药卫生—针灸推拿学]