荷斯坦奶牛红细胞作为异种血源的研究  

Holstein-Friesian RBC as Human Blood Substitute

作　　者：陈利萍[1] 李素波[2] 张雪[2] 宫锋[2] 高红伟[2] 徐华[1] 

机构地区：[1]陕西省血液中心,陕西西安710061 [2]军事医学科学院野战输血研究所血液分子生物学研究室,北京100850

出　　处：《中国实验血液学杂志》2014年第4期1099-1102,共4页Journal of Experimental Hematology

基　　金：国家自然科学基金(81300447)

摘　　要：α-Gal抗原是引起异种移植超急性排斥反应的主要异种抗原。本研究探讨新型基因重组α-半乳糖苷酶对荷斯坦奶牛红细胞上的异种抗原的作用。用酶解法清除红细胞表面的α-Gal抗原,用盐水法或抗人球蛋白法进行奶牛红细胞与人血浆的凝集试验,用流式细胞术检测奶牛红细胞表面的α-Gal抗原(FITC-IB4)标记和FITC-IgG标记红细胞的荧光强度。结果表明,盐水法和抗人球蛋白法检测显示,酶解后荷斯坦奶牛红细胞与人混合血清不发生凝集。流式细胞术检测显示,酶解后荷斯坦奶牛红细胞较酶解前其FITC-IB4标记率降低了99.0%,FITC-IgG标记率降低了87.8%。结论:新型α-半乳糖苷酶可以用来清除荷斯坦奶牛红细胞上的α-Gal抗原,且荷斯坦奶牛红细胞的异种抗原主要是α-Gal抗原。酶解法制备的荷斯坦奶牛红细胞可能成为人血替代品。α-Gal, the main xenotransplantation antigen, can lead to hyperacute rejection （HAR） in xenotransplantation. This study was purposed to investigate the effect of recombinant α-galactosidase （a-Gal antigen） on the Holstein- Friesian（H-F） red blood cells （RBC）. The enzymelysis method was used to digest the α-Gal antigen on H-F RBC; the saline and anti-human globulin methods were used to perform the agglutination test of H-F RBC and human plasma; the flow cytometry was used to detect the α--Gal antigen on surface of H-F RBC, fluorescence intensity of FITC-IB4 and FITC-IgG labeled RBC. The results indicated that the saline and anti-human globulin method showed α-galactosidase- treated H-F RBC fail to agglutinate with human pooled plasma; the flow cytometry showed the fluorescence intensity of FITC-IB4 and FITC-IgG labled RBC decrease 99.0% and 87.8%, respectively. It is concluded that the novel α-galacto- sidase can be used to cleared the α-Gal antigen on the surface of H-F RBC and α-galactosidase-treated H-F RBC may be considered as human blood substitute.

关 键 词：荷斯坦奶牛 红细胞 异种抗原 Α-半乳糖苷酶 

分 类 号：R331.141[医药卫生—人体生理学]