注射用丹酚酸A防治肝纤维化的实验研究  

Effects of salvianolic acid A injection on CCl_4 induced hepatocyte injury and hepatic fibrosis in rats

作　　者：鲁珽[1,2] 陈方明[1,2] 朱科燕[1,2] 蔡月琴[1,2] 寿旗扬[1,2] 潘永明[1,2] 陈民利[1,2] 

机构地区：[1]浙江大学医学院附属第二医院临床研究中心,杭州310009 [2]浙江中医药大学动物实验研究中心,杭州310053

出　　处：《中国比较医学杂志》2014年第7期57-62,F0004,共7页Chinese Journal of Comparative Medicine

基　　金：浙江省卫生高层次创新人才培养工程项目

摘　　要：目的探讨注射用丹酚酸A抗肝纤维化的作用,为丹酚酸A的临床应用提供理论依据。方法采用CCl4体外诱导肝细胞损伤,观察丹酚酸A对肝细胞活性及其细胞培养上清液ALT、AST、LDH水平和细胞裂解液中SOD活性和MDA含量的变化;另采用皮下注射CCl4诱导大鼠肝纤维化模型,观察丹酚酸A对肝纤维化大鼠血清LN、HA、SOD和MDA含量的影响以及肝脏组织病理改变情况。结果与模型对照组比,丹酚酸A高、低剂量组和Vit E组的细胞存活率显著提高(P<0.01),丹酚酸A高剂量组ALT、AST和LDH活性显著降低(P<0.01),丹酚酸A高剂量组和Vit E组SOD活性明显升高(P<0.05),MDA含量显著降低(P<0.05);体内试验发现,与模型对照组比,丹酚酸A高剂量组纤维化大鼠的血清LN和HA水平显著降低(P<0.05),高、低剂量组SOD活性显著升高(P<0.05,P<0.01),MDA含量显著降低(P<0.01,P<0.05),并能改善肝脏病理形态。结论注射用丹酚酸A可通过抗脂质过氧化作用,起到保护肝细胞,减轻肝纤维化的作用。Objective To study on the anti-hepatic fibrosis effects of salvianolic acid A injection （SAA）, and further to provide the theoretical basis for the clinical application .Methods Using CCl4 induced hepatocyte injury in vitro, the hepatocyte viability , the levels of ALT , AST and LDH in cell culture supernatants and the levels of SOD and MDA in cell lysates were detected .In addition , the hepatic fibrosis rat model was made by subcutaneous injection of CCl 4 , the serum LN, HA, SOD and MDA levels were detected and the pathological changes in liver tissue were also observed . Results Compared with model group , the hepatocyte viability in SAA high or low dose group and Vit E group were significantly increased （P 〈0.01）, and the activities of ALT, AST and LDH in SAA high dose group were significantly lowed （ P ＆lt;0.01 ） .The activity of SOD in SAA high dose group and Vit E group was significantly increased （ P ＆lt;0.05）, while MDA content was decreased （P ＆lt;0.05）.Vivo test showed that the levels of serum LN and HA in SAAhigh dose group were significant lower than those of hepatic fibrosis rat model group （P ＆lt;0.05）.Moreover, the activity of SOD in SAA high or low dose group was significantly increased （P ＆lt;0.05, P ＆lt;0.01）, while MDA content was lowed （P ＆lt;0.05, P ＆lt;0.01）, and can improve the pathological of liver tissues .Conclusions SAA injection can anti-lipid peroxidation and thereby protect hepatocyte and reduce hepatic fibrosis .

关 键 词：丹酚酸A 肝纤维化 脂质过氧化 四氯化碳 

分 类 号：R332[医药卫生—人体生理学]