家养野猪脑心肌炎病毒的分离、鉴定及全基因组序列分析  

Isolation,identification and full-length genome sequence of EMCV from domesticated boar

作　　者：常洪涛[1] 刘慧敏[2] 赵军[1] 陈陆[1] 杨霞[1] 王新卫[1] 姚惠霞[1] 王川庆[1] 

机构地区：[1]河南农业大学牧医工程学院,郑州450002 [2]中国科学院动物研究所,北京100101

出　　处：《中国人兽共患病学报》2014年第8期793-796,共4页Chinese Journal of Zoonoses

基　　金：国家自然科学基金(No.31272567)资助~~

摘　　要：目的：分离家养野猪脑心肌炎病毒（Encephalomyocarditis virus ，EMCV），作全基因组序列测定及与相关物种同源性比较。方法用改进的“细胞接种与RT-PCR方法相结合”技术，成功分离到国内首株家养野猪EMCV JZ1202株，并对其全基因组序列进行测定和分子特征分析。结果分离毒的基因组全长为7735 bp ，与国内外不同动物源EMCV参考毒株的核苷酸同源性为81．2％～99．9％，与国内猪源EMCV分离毒的同源性高达99．4％以上。基于EMCV全基因组、ORF和VP1基因序列绘制的系统发育进化树显示，EMCV可分为 G1、G2和G33个群，JZ1202株与其他国内参考毒株同属于G1群。结论结果证实，家养野猪可感染EMCV并引起发病，提醒作野生动物养殖要考虑EMCV的传播；EMCV存在较大的地域差异，在家养野猪和鼠之间可能存在着交叉感染；EMCV在感染家养野猪时可能发生个别氨基酸突变，以适应不同的猪种。Encephalomyocarditis virus （EMCV） ,named JZ1202 ,was isolated from domesticated boar in Henan ,China . We performed the full-length genome sequencing and molecular characteristic analysis of the isolated strain .Results showed that the full-genome sequence of EMCV JZ1202 generated a sequence of 7 735 bp in length ,and had 81 .2%-99 .9% nucleotide identity with other reference strains from different animals ,but 99 .4% with Chinese reference from pig .The phylogenetic tree was constructed based on the full-length genome;ORF and VP1 gene sequences identified EMCV was divided into G1 ,G2 and G3 groups ;the strain JZ1202 belongs to G1 with other Chinese reference strains .Results identified that the EMCV infection could cause severe clinical symptoms in domesticated boar .Big regional differences exist in EMCV and the transmission is limit-ed in a range of area .However ,cross infection and prevalence of EMCV disease between domesticated boar and mice might ex-ist .Mutation of some amino acid may occurred in EMCV infected domesticated boar .

关 键 词：脑心肌炎病毒 家养野猪 全基因组 分子特征分析 

分 类 号：R373[医药卫生—病原生物学]