湖北钉螺指名亚种与滇川亚种的趋光性比较研究(英文)  

Phototaxis effect of Oncomelania hupensis hupensis and O.h.robertsoni——a comparative study

作　　者：龚珍强 李传昌[1] 陶波 汪雁[1] 周宪民[1,3] 邹节新[1] 

机构地区：[1]南昌大学医学院寄生虫学教研室,南昌330006 [2]江西省九江市星子县血吸虫病防治站,星子332080 [3]南昌大学鄱阳湖环境与资源利用教育部重点实验室,南昌330047

出　　处：《中国人兽共患病学报》2014年第8期833-839,共7页Chinese Journal of Zoonoses

基　　金：Supported by the National Natural Science Foundation of China(Nos.81160207and 81260257);the National Undergraduate Training Programs for Innovation and Entrepreneurship(No.201310403026)~~

摘　　要：目的比较采自江西省的湖北钉螺指名亚种与四川省的湖北钉螺滇川亚种,共4个地域株的雌、雄螺及携带日本血吸虫阴、阳性螺间的趋光性差异。方法野外采集江西省日本血吸虫流行区星子县和玉山县、四川省日本血吸虫流行区天全县和彭山县的钉螺,逸蚴法筛选出阴、阳性钉螺,人工方法分离雌、雄螺,利用自制的趋光性实验暗箱,以白色光为光源,最适宜光照强度下分别对4个地域株的雌、雄螺及阴、阳性螺进行趋光性实验。结果在1 500Lux的光照强度下,30min实验时间内,8个阴性螺实验组均表现出明显的趋光性;经统计分析,雌、雄钉螺间趋光指数无显著性差异;同一亚种的不同地域株钉螺之间的趋光指数无显著性差异;2个亚种间的钉螺在5、10、15、20、25min时趋光指数无显著性差异;在30min时2个亚种间的趋光指数具有显著性差异,且指名亚种高于滇川亚种;日本血吸虫阳性钉螺在30min的趋光性显著低于阴性螺。结论湖北钉螺不同亚种间的趋光性存在差异性,而同一亚种内的所有阴性钉螺间的趋光性相同,当其感染了日本血吸虫后趋光性降低。The aim of this study was to determine the differences in phototaxis between Oncomelania hupensis （O .h .） hupensis and O .h .robertsoni as well as four related geographic strains of male and female snails and Schistosoma japonicum-infected and-uninfected snails .Field collection of O . hupensis was performed in S . j aponicum-endemic areas of Xingzi and Yushan counties in Jiangxi Province ,China ,as well as Tianquan and Pengshan counties in Sichuan Province ,China .Infected and uninfected snails were screened using the cercariae escaping method ,with artificial separation of male and female snails . The experiment was carried out in an in-house constructed dark phototaxis experiment box using white light as the main source of light at the most suitable light intensity .Under a light intensity of 1 500 Lux for 30 minutes ,all of the snails from the experimental group showed obvious phototaxis .The statistical analysis showed no significant difference in the phototaxis index between male and female snails ,and there was also no significant difference in the phototaxis index between different geograph-ical strains of the same subspecies of snails .A significant difference in phototaxis was observed between the two subspecies during the 30-minute experimental period ：O .h .hupensis displayed a higher phototaxis index than that of O .h .robertsoni , whereas S .japonicum-infected snails showed a lower phototaxis index than that of uninfected snails .A difference in phototaxis was found between different subspecies of O .hupensis and within the same subspecies .All uninfected snails displayed the same phototaxis index .When snails were infected with S .japonicum ,phototaxis reduced .

关 键 词：湖北钉螺 日本血吸虫 趋光性 光照强度 

分 类 号：R383.2[医药卫生—医学寄生虫学]