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机构地区:[1]暨南大学第二附属医院(深圳市人民医院)检验科,深圳518020
出 处:《中国人兽共患病学报》2014年第8期866-870,共5页Chinese Journal of Zoonoses
基 金:深圳市科技创新委员会基金(No.CXB201111250114A)资助~~
摘 要:基质辅助激光解吸/电离飞行时间质谱(Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry,MALDI-TOF MS)技术是近年发展起来的一种软电离新型有机质谱,它可以在几分钟内直接对平皿中或标本中的菌落进行鉴定。这种新而简单的方法大大降低了耗材成本和鉴定诊断时间。可靠性和准确性已在许多研究报告中得以证明,不同的系统设备也已在市场销售,在不久的将来,该项技术会在实际应用中拥有更加广阔的前景。MALDI-TOF MS将会成为替代传统人类病原体微生物鉴定手段的革新技术。本文将回顾MALDI-TOF MS的原理、发展、应用情况、与传统方法相比的优势及现阶段存在的局限性,着重强调该项技术在微生物系统的应用特点。In recent years ,matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF MS) is a new organic soft ionization mass spectrometry which can identify and classify microorganism by directly using the sam-ple itself or cultivate colonies on petri dish in a few minutes .This new and simple method reduces the cost of consumables and the time of diagnosis greatly .Its reliability and accuracy have been shown in many studies and different system devices have al-ready been on the market .In the near future ,MALDI-TOF MS will have a broader prospect .This mass spectrometry will be-come an effective and fast microbial identification technology to replace the conventional method .In this article ,we will review the principles of MALDI-TOF MS ,its development and application ,its advantages and shortcomings which compared with tra-ditional methods at the present stage ,and the emphasis will focus on the application characteristics of MALDI-TOF MS in mi-crobial systems .
分 类 号:R372[医药卫生—病原生物学]
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