感染减毒鼠伤寒沙门氏菌对小鼠粪样代谢组的影响——WIPM和Bruker 500 MHz核磁共振波谱仪检测结果的比较  

Effects of Attenuated Salmonella Typhimurium Infection on Fecal Metabonome in Mice——comparison between WIPM and Bruker 500 MHz NMR spectrometers

作　　者：陈璐[1,2] 宋侃[1,3] 王玉兰[1] 

机构地区：[1]中国科学院生物磁共振分析重点实验室,波谱与原子分子物理国家重点实验室,武汉磁共振中心(中国科学院武汉物理与数学研究所),湖北武汉430071 [2]长江航务管理局疾病预防控制中心,湖北武汉430019 [3]中国科学院大学,北京100049

出　　处：《波谱学杂志》2014年第3期349-363,共15页Chinese Journal of Magnetic Resonance

基　　金：国家重大科学仪器设备开发专项资助项目(2011YQ12003503)

摘　　要：鼠伤寒沙门氏菌属于胞内侵袭性兼性厌氧菌,减毒后的鼠伤寒沙门氏菌安全性大大提高,已被广泛用于病毒、细菌及寄生虫疫苗的研制.但是目前还没有研究利用代谢组学方法来分析减毒鼠伤寒沙门氏菌感染对宿主和肠道菌群相互作用的影响.同时中国科学院武汉物理与数学研究所自主研发的WIPM 500 MHz核磁共振(NMR)波谱仪已经研制成功,但是该谱仪是否可以用于代谢组学研究尚未经过考证.本研究同时使用WIPM和Bruker500 MHz核磁共振波谱仪对同一批减毒鼠伤寒沙门氏菌感染小鼠的粪样进行检测,并结合多变量数据分析方法来研究减毒鼠伤寒沙门氏菌感染对小鼠粪样代谢组的影响.研究结果表明,WIPM NMR波谱仪检测数据的分析结果与Bruker NMR波谱仪的实验结果具有高度的一致性,表明WIPM谱仪可以用于代谢组学研究.研究发现减毒鼠伤寒沙门氏菌感染能够引起小鼠粪样代谢物中多种氨基酸和尿嘧啶含量的上升,并伴有胆汁酸、乳酸和丙酸含量的下降.以上代谢物含量的改变表明,减毒鼠伤寒沙门氏菌感染使小鼠肠道微生物的代谢功能发生了紊乱.Salmonella typhimurium is a intracellular, invasive, facultative anaerobic bacteria. With improved safety, attenuated salmonella typhimurium has been widely used in development of vaccine for preventing viruses, bacteria, and parasites infections. However, limited research has been done so far on the biological effects of attenuated salmonella typhimurium infection on animal hosts. In this work, we measured fecal metabonome of mice infected with attenuated salmonella typhimurium using NMR spectroscopy. Two sets of NMR data were acquired with the same protocols on a Bruker 500 MHz NMR spectrometer and an in-house 500 MHz NMR spectrometer developed in Wuhan Institute of Physics and Mathematics （WIPM）. Multivariate data analysis methods were used to analyzed the data. It was found that infection induced increases of certain amino acids and uracil in feces, and decreases of bile acids, lactate and propionate. Such metabolic alterations indicated that infection of attenuated salmonella typhimurium may cause disturbance of gut microbiotal communities. The results obtained from the home-developed spectrometer in WIPM showed high consistency with those obtained from Bruker 500 MHz NMR spectrometer, suggesting that the WIPM spectrometer is ready for metabonomic studies.

关 键 词：核磁共振(NMR) 粪样代谢组 减毒鼠伤寒沙门氏菌 谱仪对比 

分 类 号：O482.53[理学—固体物理]