七味白术散对相关肠道病原体感染HT-29细胞IP-10表达的影响  

Effect of Qiwei Baizhu powder on IP-10 expression in HT-29 cells infected with enteric pathogens

作　　者：湛蓝[1] 伍参荣[1] 谭晓凤[1] 刘卫东[1] 孙必强[1] 周英[1] 蔡锐[1] 李杰[1] 李珊[1] 

机构地区：[1]湖南中医药大学,湖南长沙410208

出　　处：《湖南中医杂志》2014年第8期152-154,共3页Hunan Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金资助项目(编号:81273990);国家教育部博士导师基金资助项目(编号:2013000011);湖南省中医药管理局重点资助项目(编号:1300001)

摘　　要：目的:观察七味白术散对轮状病毒(HRV)、柯萨奇B3病毒(Cox B3)感染HT-29细胞干扰素诱导蛋白-10(IP-10)表达的影响。方法:将HT-29细胞株分别随机设成正常组、模型组、七味白术散组、病毒唑组,于37℃、5%CO2条件下培养48h,收获培养物。采用RT-PCR技术测定IP-10mRNA的表达情况。结果:两种肠道病原体感染HT-29细胞后,与正常组比较,模型组IP-10mRNA的表达明显升高(P<0.05);与模型组比较,七味白术散组和病毒唑组的IP-10mRNA表达明显降低(P<0.05);七味白术散组表达值低于病毒唑组(P<0.05)。结论:七味白术散能下调HRV、COX B3感染HT-29细胞的IP-10的表达。Objective：To observe the effect of Qiwei Baizhu powder on the expression of interferon - inducible protein 10 （ IP - 10） in HT - 29 cells infected with human rotavirus （HRV） and coxsaekievirus B3 （ Cox B3 ）. Methods ： HT - 29 ceils were randomly divid- ed into normal group, model group, Qiwei Baizhu powder group, and virazole group and were cultured at 37~C and 5% CO2 for 48 h. The nlRNA expression of IP - 10 was measured by RT - PCR. Results：After being infected with the two enteric pathogens,the model group had significantly increased IP - 10 mRNA expression compared with the normal group （ P 〈 0. 05 ） ; the Qiwei Baizhu powder group and virazole group had significantly reduced IP - 10 expression compared with the model group （ P 〈 0. 05 ） , and the Qiwei Baizhu powder group had a significantly lower IP - 10 expression than the virazole group （P 〈 0. 05 ）. Conclusion：Qiwei Baizhu powder can downregulate the IP- 10 expression in HT- 29 cells infected with HRV and COX B3.

关 键 词：七味白术散 人轮状病毒 柯萨奇B3病毒 IP-10 

分 类 号：R285.5[医药卫生—中药学]