蕨麻正丁醇提取物对卵巢缺血再灌注损伤大鼠内皮素-1的影响  

Effect of Potentilla anserina on endothelin-1 in acute ovary ischemia reperfusion injury in rats

作　　者：邹晓萍[1] 董化江[2] 单娜娜[3] 臧照辉[4] 单云官[2] 

机构地区：[1]武警后勤学院附属医院妇产科,天津300162 [2]武警后勤学院人体解剖学与组织胚胎学教研室天津市遗体防腐与整形整容重点实验室,天津300162 [3]武警后勤学院教育技术中心,天津300162 [4]武警后勤学院研究生处,天津300162

出　　处：《新乡医学院学报》2014年第9期682-684,共3页Journal of Xinxiang Medical University

基　　金：国家十一五科技支撑计划项目(编号:2007BAK38B05);武警后勤学院科研创新团队项目(编号:WHTD201308-2)

摘　　要：目的探讨蕨麻正丁醇(Pa)对急性卵巢缺血再灌注损伤大鼠血清及卵巢组织中内皮素-1(ET-1)的影响。方法 Wistar雌性大鼠60只,随机分为6组:假手术组(C组)、缺血24 h组(I24h)、缺血再灌注24 h组(R24h组)、缺血再灌注48 h组(R48h组)、给予Pa提取物预处理+缺血再灌注24 h组(Pa+R24h组)和Pa提取物预处理+缺血再灌注48 h组(Pa+R48h组)。酶联免疫吸附试验测定法检测各组血清ET-1水平;反转录聚合酶链反应、Western blot法检测各组大鼠卵巢组织中ET-1 mRNA和蛋白的表达。结果与C组比较,I24h组、R24h组和R48h组大鼠血清ET-1水平、卵巢组织中ET-1 mRNA及蛋白表达均显著升高(P<0.05);与I24h组比较,R24h组、R48h组大鼠血清ET-1水平、卵巢组织中ET-1 mRNA和蛋白表达均显著升高,差异有统计学意义(P<0.05)。而Pa+R24h组和Pa+R48h组大鼠血清ET-1水平、卵巢组织中ET-1 mRNA和蛋白表达与R24h组、R48h组比较显著下降,差异有统计学意义(P<0.05)。结论 ET-1在大鼠卵巢缺血再灌注损伤过程中表达上调,Pa提取物进行干预可降低ET-1的表达。Objective To investigate the effect of Potentilla anserina（Pa） on endothelin-1 （ET-1） in acute ovary ischemia reperfusion injury in rats. Methods Sixty female Wistar rats were randomly divided into sham group （ group C） ,ischemic 24 hours group （ group I24h）, ischemic reperfusion 24 hour group （ group R24h）, ischemic reperfusion 48 hour group （group R48h） ,Pa ＋ ischemic reperfusion 24 hour group（ Pa ＋ R24h） and Pa ＋ ischemic reperfusion 48 hour group（ Pa ＋ R48h）. The serum level of ET-1 was determinated by enzyme linked immunosorbent assay. Reverse transcriptase polymerase chain reaction and Western blot were used to detect the ET-1 mRNA and protein in ovary tissue of rats. Results Compared with group C,ET-1 level in serum,ET-1 mRNA and protein in ovary tissue in group I24h,R24h and R48h increased significantly （P 〈 0. 05 ） ;Compared with group I24h, ET-1 level in serum, ET-1 mRNA and protein in ovary tissue in group R24h and R48h increased significantly（P 〈 0.05）. The ET-1 level in serum,ET-1 mRNA and protein in ovary tissue in group Pa ＋ R24b and Pa ＋ R48h decreased significantly compared with group R24h and R48h （ P 〈 0.05 ）. Conclusion ET-1 level is increasing in ovary ischemia and reperfusion injury in rats, which can be reduced by Pa.

关 键 词：蕨麻正丁醇 大鼠 卵巢缺血再灌注损伤 内皮素-1 

分 类 号：R285.5[医药卫生—中药学]