商陆皂甙甲对大鼠的体外杀精作用  

Spermicidal effect of esculentoside A on rats in vitro

作　　者：易文龙[1] 郭晓轶 樊舒豪 谌曼荣 王育斌[2] 

机构地区：[1]武汉科技大学附属天佑医院儿科,湖北武汉430064 [2]武汉科技大学医学院生理学系,湖北武汉430065

出　　处：《新乡医学院学报》2014年第9期688-690,共3页Journal of Xinxiang Medical University

基　　金：国家级大学生创新训练项目(编号:201210488036);武汉市科学技术计划项目(编号:201051299451)

摘　　要：目的探讨商陆皂甙甲(EsA)对大鼠的体外杀精作用及其机制。方法采用手术方法采集Sprague-Dawley大鼠精子,随机分为EsA组、生理盐水组及壬苯醇醚-9(N-9)组,采用改良Sander-Cramer法,将不同浓度EsA制剂在体外与大鼠精子作用,测定EsA的杀精作用及杀精最低有效浓度;精子尾低渗肿胀(HOS)试验、伊红Y(EY)染色法检测精子膜的完整性和活力,固相法检测精子顶体酶(ACE)活性。结果作用20 s,最低有效质量浓度为2.0 g·L-1的EsA对大鼠精子具有快速杀精作用;与生理盐水组比较,最低有效质量浓度(2.0 g·L-1)的EsA作用20 s后,精子尾肿胀率显著下降(P<0.05),EY染色率显著升高(P<0.05);与生理盐水组比较,EsA各浓度组ACE活性均显著下降(P<0.05),且药物浓度越大,ACE活性降低越明显,组间两两比较差异均有统计学意义(P<0.05)。结论 EsA对大鼠有体外杀精作用,其机制可能与破坏精子膜和降低ACE活性有关。Objective To study the spermicidal effect of esculentoside A （EsA） on rats in vitro and its mechanism. Methods The Sprague-Dawley rats＇ sperms were collected by operation method, which were randomly divided into EsA group, normal saline （NS） group and nonoxinol-9 （N-9） group. The different concentration EsA acted on rats＇ sperms in vitro, and the modified Sander-Cramer assayed was used to detect the spermicidal effect and spermicidal minimum effective concentration （MEC）. The hypo-osmotic swelling （HOS） test and the eosin Y （EY） staining were used to detect the integrity of sperm membrane and vitality, the solid-phase method was used to detect the acrosomal enzyme （ACE） activity. Results MEC of EsA at 20 s was 2.0 g·L^ - 1. Compared with NS group, the rate of the tails swollen sperm was significantly reduced in EsA groups （ P 〈 0.05 ）, the rate of stained sperm was significantly increased in EsA groups （P 〈 0.05 ）, the activity of ACE were all significantly reduced in EsA groups （P 〈 0.05 ）, and the reducing was associated with drug concentration, the differences were statistically significant by pairwise comparison（ P 〈 0.05 ）. Conclusion EsA has spermicidal effect on rats in vitro, the spermicidal effect may occur by the disruption of the sperm membrane integrity and the reducing of the ACE activity.

关 键 词：商陆皂甙甲 体外杀精 低渗肿胀试验 精子顶体酶 

分 类 号：R285[医药卫生—中药学]