埃克替尼对ACC-M细胞凋亡及Bcl-2,Bax蛋白表达的影响  

Effect of Icotinib on the Apoptosis of ACC-M Cells and the Protein Expression of Bcl-2 and Bax

作　　者：杨彩玲[1] 韩金红[2] 王国戗[1] 黄艳梅[2] 路德荣 崔卫刚[2] 

机构地区：[1]新乡医学院第一附属医院,河南新乡453003 [2]新乡医学院基础医学院,河南新乡453003 [3]新乡医学院第三附属医院,河南新乡453003

出　　处：《河南师范大学学报（自然科学版）》2014年第4期125-128,共4页Journal of Henan Normal University(Natural Science Edition)

基　　金：新乡医学院高学历人才资助课题(GXLZZ2008-09)

摘　　要：按照0(对照组)、10、20、40μmol·L-1浓度配制盐酸埃克替尼溶液,分别作用于人涎腺腺样囊性癌细胞株(ACC-M)24、48、72h,用流式细胞仪测药物作用后ACC-M细胞凋亡率;同时利用免疫细胞化学方法和间接免疫荧光联合流式细胞技术,检测它在体外对ACC-M细胞Bcl-2、Bax蛋白表达的影响.结果显示,细胞凋亡率随盐酸埃克替尼浓度与作用时间的增加而增加,经统计学分析,细胞凋亡率与盐酸埃克替尼浓度、作用时间成正相关,药物作用于ACC-M细胞后Bcl-2蛋白表达降低,Bax蛋白表达增加.实验表明,盐酸埃克替尼可能通过降低Bcl-2蛋白的表达,增加Bax蛋白的表达,诱导ACC-M细胞凋亡.In this study, we used different concentrations of Icotinih hydrochloride [respectively 0 （for control group）, 10, 20 and 40 μmol·L-1 to act on ACC-M cells of human beings for respectively 24, 48, and 72 h. And then we tested the apoptosis rate with flow cytometer. In the meantime, we used the techniques of immunocytochemistry and indirect immunofluo- rescence to test the effect of Icotinib upon the protein expression of Bcl-2 and Bax in ACC-M cells in vitro. The tests showed that the apoptosis rate increased with the enhancement of the consistency of Icotinib hydrochloride and the amount of time used. Statistical analysis showed that there was a positive correlation between the apoptosis rate and the consistency of Icotinib hydro chloride and the amount of time used in the process, and that the protein expression of Bcl-2 decreased while that of Bax in- creased under the influence of Icotinib. Our experiment shows that Icotinib hydrochloride may induce the apoptosis of ACC-M ceils via reducing the expression of Bcl-2 and increasing that of Bax.

关 键 词：埃克替尼 ACC-M细胞 凋亡 BCL-2 BAX 

分 类 号：Q257[生物学—细胞生物学]