HPCE法检测齐考诺肽的含量  

Determination of Ziconotide by HPCE

作　　者：陈玉娟[1] 凡炼炼[2] 于源华[1] 

机构地区：[1]长春理工大学生命科学技术学院,长春130022 [2]吉林大学中日联谊医院,长春130033

出　　处：《长春理工大学学报（自然科学版）》2014年第4期161-163,共3页Journal of Changchun University of Science and Technology(Natural Science Edition)

基　　金：吉林省科技厅重点科技攻关项目(20140204052YY)

摘　　要：通过高效毛细管电泳法（HPCE）,建立一种检测齐考诺肽含量的方法。本文利用线性、精密度、稳定性、重现性、加样回收率等实验,利用HPCE法检测合成的齐考诺肽样品中齐考诺肽的含量,测定齐考诺肽在100-500mg/mL（r=0.999）范围内成良好的线性关系,精密度、重现性、稳定性、加样回收率等实验的RSD值均小于3%,加样回收率平均值为100.18%。本文首次利用HPCE法建立了检测齐考若肽含量的测定方法。结果表明此法线性好、精密度高、重现性好、结果准确,前处理简单;HPCE操作简单,分析速度快,适合齐考诺肽的含量检测。To establish a new method for determination of ziconotide,high performance capillary electrophoresis（HPCE）was employed. HPCE was used to determine the content of ziconotide by experiments as linearity,precision,reproducibility and recoveries. The linear ranges of Ziconotide was 10-150ng/mL（r =0.9999）by HPCE,the RSD of precision,reproducibility,stability and recoveries were all less than 3%. And the average recovery by HPCE was 100.18%.A new method was established to determine Ziconotide. The method is simple,rapid,reproducible,accurate,and with good linearity,high precision. So HPCE is more suitable for content determination of Ziconotide.

关 键 词：齐考诺肽 高效毛细管电泳 含量测定 

分 类 号：R917[医药卫生—药物分析学]