硫酸苯酚法测定燕窝及常见伪品中多糖含量  

Phenol-Sulphuric Acid Method for the Determination of Reductive Sugar Content of Edible Bird's Nest

作　　者：李耿[1] 梁月亮 张健润[1] 苏振宁 戴洁[1] 刘鹏[1] 赖小平[3] 

机构地区：[1]广州中医药大学中药学院,广州510006 [2]广州同康药业有限公司,广州511356 [3]东莞广州中医药大学中医药数理工程研究院,东莞523000

出　　处：《中国中医药现代远程教育》2014年第15期148-149,共2页Chinese Medicine Modern Distance Education of China

基　　金：国家自然科学基金面上项目[No:81173498];广东省高等学校珠江学者岗位计划资助项目(2011年度);粤港澳岭南中药综合开发研究[No:2014DFH30010]

摘　　要：目的建立燕窝中燕窝多糖的含量测定方法。方法采用紫外分光光度法,苯酚-浓硫酸比色法于波长492nm处测定吸光度。结果标准曲线的线性范围为0~30mg/mL（r=0.9997）,平均加样回收率为99.61%,RSD=1.60%。结论该方法简便、快速、准确,可用于燕窝中燕窝多糖的含量测定。Objective To establish a method for content determination of bird＇s nest polysaccharides in the bird＇s nest.Methods UV spectrophotometry,phenol-sulfuric acid at the wavelength of 492 nm absorbance.Results The linear range of total bird＇s nest polysaccharides was 0~30mg/mL（r=0.9997）.The recovery rate of the study was 99.61%,RSD=1.60%.Conclusion The results of simulation indicate that this method was accurate,reliable and reproducible.It was suggested that this method can be used to determine the content of bird＇s nest polysaccharides in the bird＇s nest.

关 键 词：燕窝 多糖 含量测定 紫外分光光度法 硫酸苯酚法 

分 类 号：R284[医药卫生—中药学]