新基因NtTKR及其同源物LeTKR的生物信息学分析  

Bioinformatics Analysis of a New Gene NtTKRand its Homologue LeTKR

作　　者：李芬[1,2] 吴秀丽[1] 郭君丽[1] 

机构地区：[1]河南师范大学生命科学学院,河南新乡453007 [2]河南师范大学资源微生物与功能分子河南省高校重点实验室培育基地,河南新乡453007

出　　处：《河南师范大学学报（自然科学版）》2014年第5期110-115,124,共7页Journal of Henan Normal University(Natural Science Edition)

基　　金：国家自然科学基金(30600343);河南省高校科技创新团队支持计划资助(13IRTSTHN009)

摘　　要：驱动蛋白是一类与微管结合的ATPase,在细胞内执行着多种功能.序列对比分析表明,烟草不同发育时期胚cDNA文库的差异筛选分离到的在叶和发育各时期胚中优势表达的驱动蛋白家族新成员NtTKR与番茄驱动蛋白LeTKR(AF242356.1)高度同源.运用生物信息学方法对二者编码产物的同源性、功能结构域特点和可能的翻译后修饰等进行了比较分析,结合目前已知的实验结果预测了该基因可能参与的生物学过程.这些分析和预测将对进一步实验证实NtTKR在烟草的形态发生及胚胎发生中的提供有益的指导.Kinesin, a kind of ATPase binding with microtube, has many functions in cell. A new member of Kinesin family, NtTKR, expressed in leaves and embryos in every different development stage, was isolated by screening eDNA library of embryo, has the most similar sequence to Lycopersicon esculintum kinesin related protein （AF242356.1）. Based on the comparison analysis of their homology, functional domains and possible posttranslational modification of their products, with results reported, we predicted the possible processes in which NtTKR involved. This information will provide directions for further studies to verify the possible roles of NtTKR in embryogenesis and leaf morphogenesis.

关 键 词：烟草 驱动蛋白(Kinesin) 生物信息学分析 形态发生 

分 类 号：Q71[生物学—分子生物学] R329.2[医药卫生—人体解剖和组织胚胎学]